Team:Caltech/Notebook

From 2014.igem.org

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<tr><td colspan="3"> <h3>Notebook</h3></td></tr>
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<tr><td colspan="3"> <h3>Notebook</h3> Apparently there's a calendar feature to take care of this too???</td></tr>
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<tr><td colspan=3><h1>Week One</h1></td></tr>
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<p>You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well. </p>
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<h4>Tuesday, 6/17/14</h4>
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<ul><li>Get acquainted with summer logistics and lab basics</li>
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    <li>Clean up lab space, stock lab supplies</li>
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</ul>
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<td>We got absolutely no results today!!!</td>
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<h4>Wednesday, 6/18/14</h4>
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<ul><li>Continue to discuss project and clean up lab space</li>
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    <li>Designed PCR primers for combinatorial promoters subproject</li>
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</ul>
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</td>
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<td>We got absolutely no results today!!!</td>
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<h4>Thursday, 6/19/14</h4>
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<ul><li>Formulated plasmid design for the response regulation subproject</li>
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    <li>PCR plasmid backbone and sfGFP to create constructs with the proper overhangs for Gibson assembly</li>
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    <li>Gel electrophoresis for confirmation of PCR products' length</li>
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</ul>
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</td>
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<td>We got absolutely no results today!!!</td>
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<h4>Friday, 6/20/14</h4>
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<ul><li>Purification of PCR products created yesterday</li>
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    <li>Gibson assembly of purified products</li>
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    <li>Transformation of Gibson-assembled plasmids into JM109 E. coli</li>
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    <li>Meeting with Prof. Murray to discuss give an update on the project</li>
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</ul>
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<td>Colonies were found after transformation into </td>
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Revision as of 04:41, 23 June 2014



WELCOME TO iGEM 2014!

Your team has been approved and you are ready to start the iGEM season!
On this page you can document your project, introduce your team members, document your progress
and share your iGEM experience with the rest of the world!


Click here to edit this page!

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Notebook

Apparently there's a calendar feature to take care of this too???

Week One

Tuesday, 6/17/14
  • Get acquainted with summer logistics and lab basics
  • Clean up lab space, stock lab supplies
 We got absolutely no results today!!!

Wednesday, 6/18/14
  • Continue to discuss project and clean up lab space
  • Designed PCR primers for combinatorial promoters subproject
 We got absolutely no results today!!!

Thursday, 6/19/14
  • Formulated plasmid design for the response regulation subproject
  • PCR plasmid backbone and sfGFP to create constructs with the proper overhangs for Gibson assembly
  • Gel electrophoresis for confirmation of PCR products' length
 We got absolutely no results today!!!

Friday, 6/20/14
  • Purification of PCR products created yesterday
  • Gibson assembly of purified products
  • Transformation of Gibson-assembled plasmids into JM109 E. coli
  • Meeting with Prof. Murray to discuss give an update on the project
 Colonies were found after transformation into

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