Team:Bielefeld-CeBiTec/Results/CO2-fixation
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The second module aims to change E.coli in a way that it binds carbon dioxide like an autotroph organism. For this we want to establish the Calvin-cycle. E.coli has all enzymes for the Calvin-cycle except of three.<br> | The second module aims to change E.coli in a way that it binds carbon dioxide like an autotroph organism. For this we want to establish the Calvin-cycle. E.coli has all enzymes for the Calvin-cycle except of three.<br> | ||
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<div class="element" style="margin:10px; padding:10px; text-align:center; width:450px"> | <div class="element" style="margin:10px; padding:10px; text-align:center; width:450px"> | ||
<a href="https://static.igem.org/mediawiki/2014/5/54/Bielefeld-CeBiTec_2014-10-11_Carboxy_weiss_wiki.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/5/54/Bielefeld-CeBiTec_2014-10-11_Carboxy_weiss_wiki.png" width="450px"></a><br> | <a href="https://static.igem.org/mediawiki/2014/5/54/Bielefeld-CeBiTec_2014-10-11_Carboxy_weiss_wiki.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/5/54/Bielefeld-CeBiTec_2014-10-11_Carboxy_weiss_wiki.png" width="450px"></a><br> | ||
<font size="1" style="text-align:center;">Missing enzymes in Calvin-cycle</font> | <font size="1" style="text-align:center;">Missing enzymes in Calvin-cycle</font> | ||
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The different results for all three enzymes are mentioned in the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/Calvin-Cycle" target="_blank">Calvin-cycle</a> section. One important step for the carbon dioxide fixation is the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/RuBisCO" target="_blank">RuBisCO</a> (Ribulose 1,5-bisphosphate carboxylase/oxygenase). For E.coli we decided to transform genes for the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/Carboxysome" target="_blank">carboxysome</a> because of its properties. | The different results for all three enzymes are mentioned in the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/Calvin-Cycle" target="_blank">Calvin-cycle</a> section. One important step for the carbon dioxide fixation is the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/RuBisCO" target="_blank">RuBisCO</a> (Ribulose 1,5-bisphosphate carboxylase/oxygenase). For E.coli we decided to transform genes for the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/Carboxysome" target="_blank">carboxysome</a> because of its properties. | ||
Revision as of 19:31, 11 October 2014
CO2 fixation
The second module aims to change E.coli in a way that it binds carbon dioxide like an autotroph organism. For this we want to establish the Calvin-cycle. E.coli has all enzymes for the Calvin-cycle except of three.
Here you will find information about the execution of our experiments.