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Team:ETH Zurich/modeling/parameters
From 2014.igem.org
(Difference between revisions)
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|α<sub>LuxR</sub>||60 nMmin<sup>-1</sup>||Production rate of LuxR||Estimated | |α<sub>LuxR</sub>||60 nMmin<sup>-1</sup>||Production rate of LuxR||Estimated | ||
|- | |- | ||
| - | |k<sub>RLux</sub>||0.1 nM<sup>-1</sup>min<sup>-1</sup>|| Rate of formation of RLux from | + | |k<sub>RLux</sub>||0.1 nM<sup>-1</sup>min<sup>-1</sup>|| Rate of formation of RLux from Lux-AHL and LuxR||Weber |
|- | |- | ||
|k<sub>-RLux</sub>||10 min<sup>-1</sup>||Dissociation rate of RLux ||Weber | |k<sub>-RLux</sub>||10 min<sup>-1</sup>||Dissociation rate of RLux ||Weber | ||
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|K<sub>dPLux</sub>||0.007652 nM||Dissociation rate constant for DRLux-PLux ||Fitted | |K<sub>dPLux</sub>||0.007652 nM||Dissociation rate constant for DRLux-PLux ||Fitted | ||
|- | |- | ||
| - | |d<sub> | + | |d<sub>Lux-AHL</sub>||0.004 min<sup>-1</sup>||Degradation rate of Lux-AHL (30C6HSL)||Estimated/Fitted |
|- | |- | ||
|d<sub>LuxR</sub>||0.0174 min<sup>-1</sup>||Degradation rate of LuxR||Weber | |d<sub>LuxR</sub>||0.0174 min<sup>-1</sup>||Degradation rate of LuxR||Weber | ||
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|α<sub>LasR</sub>||60 nMmin<sup>-1</sup>||Production rate of LasR||Estimated | |α<sub>LasR</sub>||60 nMmin<sup>-1</sup>||Production rate of LasR||Estimated | ||
|- | |- | ||
| - | |k<sub>RLas</sub>||0.1 nM<sup>-1</sup>min<sup>-1</sup>|| Rate of formation of RLas from | + | |k<sub>RLas</sub>||0.1 nM<sup>-1</sup>min<sup>-1</sup>|| Rate of formation of RLas from Las-AHL and LasR||Weber |
|- | |- | ||
|k<sub>-RLas</sub>||10 min<sup>-1</sup>||Dissociation rate of RLas ||Weber | |k<sub>-RLas</sub>||10 min<sup>-1</sup>||Dissociation rate of RLas ||Weber | ||
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|K<sub>dPLas</sub>||1.3672 nM||Dissociation rate constant for DRLas-PLas ||Fitted | |K<sub>dPLas</sub>||1.3672 nM||Dissociation rate constant for DRLas-PLas ||Fitted | ||
|- | |- | ||
| - | |d<sub> | + | |d<sub>Las-AHL</sub>||0.004 min<sup>-1</sup>||Degradation rate of Las-AHL (30C12HSL)||Estimated/Fitted |
|- | |- | ||
|d<sub>LasR</sub>||0.0174 min<sup>-1</sup>||Degradation rate of LasR||Weber | |d<sub>LasR</sub>||0.0174 min<sup>-1</sup>||Degradation rate of LasR||Weber | ||
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|d<sub>LasI</sub>||0.0167 min<sup>-1</sup>||Degradation rate of LasI||Weber | |d<sub>LasI</sub>||0.0167 min<sup>-1</sup>||Degradation rate of LasI||Weber | ||
|- | |- | ||
| - | |k<sub> | + | |k<sub>Lux-AHL</sub>||0.04 min<sup>-1</sup>||Production rate of Lux-AHL (30C6HSL) from the LuxI||Weber |
|- | |- | ||
| - | |k<sub> | + | |k<sub>Las-AHL</sub>||0.04 min<sup>-1</sup>||Production rate of Las-AHL (30C12HSL) from the LasI||Weber |
|} | |} | ||
<html></article></html> | <html></article></html> | ||
{{:Team:ETH Zurich/tpl/foot}} | {{:Team:ETH Zurich/tpl/foot}} | ||
Revision as of 19:00, 11 October 2014
Parameters
No model is complete without parameters. Our exhaustive list of parameters are summarised in the table below.
| Parameter | Value | Description | Reference |
|---|---|---|---|
| αLuxR | 60 nMmin-1 | Production rate of LuxR | Estimated |
| kRLux | 0.1 nM-1min-1 | Rate of formation of RLux from Lux-AHL and LuxR | Weber |
| k-RLux | 10 min-1 | Dissociation rate of RLux | Weber |
| kDRLux | 0.05 nM-1min-1 | Dimerization rate of RLux | Weber |
| k-DRLux | 1 min-1 | Dissociation rate of DRLux | Weber |
| KdPLux | 0.007652 nM | Dissociation rate constant for DRLux-PLux | Fitted |
| dLux-AHL | 0.004 min-1 | Degradation rate of Lux-AHL (30C6HSL) | Estimated/Fitted |
| dLuxR | 0.0174 min-1 | Degradation rate of LuxR | Weber |
| dRLux | 0.0174 min-1 | Degradation rate of RLux | Weber |
| dDRLux | 0.0231 min-1 | Degradation rate of DRLux | Weber |
| dBxb1 | 0.01 min-1 | Degradation rate of Bxb1 | Assumed |
| LPLux | 0.04458 nM-1min-1 | Leakiness after using riboswitch for PLux | Fitted |
| αLasR | 60 nMmin-1 | Production rate of LasR | Estimated |
| kRLas | 0.1 nM-1min-1 | Rate of formation of RLas from Las-AHL and LasR | Weber |
| k-RLas | 10 min-1 | Dissociation rate of RLas | Weber |
| kDRLas | 0.05 nM-1min-1 | Dimerization rate of RLas | Weber |
| k-DRLas | 1 min-1 | Dissociation rate of DRLas | Weber |
| KdPLas | 1.3672 nM | Dissociation rate constant for DRLas-PLas | Fitted |
| dLas-AHL | 0.004 min-1 | Degradation rate of Las-AHL (30C12HSL) | Estimated/Fitted |
| dLasR | 0.0174 min-1 | Degradation rate of LasR | Weber |
| dRLas | 0.0174 min-1 | Degradation rate of RLas | Weber |
| dDRLas | 0.0231 min-1 | Degradation rate of DRLas | Weber |
| dϕc31 | 0.01 min-1 | Degradation rate of ϕC31 | Assumed |
| LPLux | 0.02461 nM-1min-1 | Leakiness after using riboswitch for PLas | Fitted |
| kDBxb1 | 1 nM-1min-1 | Dimerization rate of Bxb1 | Fitted |
| k-DBxb1 | 10-4 min-1 | Dissociation rate of DBxb1 | Fitted |
| kSABxb1 | 1 nM-1min-1 | Rate of formation of SABxb1 from DBxb1 and SIBxb1 | Fitted |
| k-SABxb1 | 10-4 min-1 | Dissociation rate of SABxb1 | Fitted |
| dDBxb1 | 0.02 min-1 | Degradation rate of DBxb1 | Assumed |
| kϕc31 | 1 nM-1min-1 | Dimerization rate of ϕc31 | Fitted |
| k-Dϕc31 | 10-4 min-1 | Rate of dissociation of Dϕc31 | Fitted |
| kSAϕc31 | 1 nM-1min-1 | Rate of formation of SAϕc31 from Dϕc31 and SIϕc31 | Fitted |
| k-SAϕc31 | 10-4 min-1 | Rate of dissociation of SAϕc31 | Fitted |
| dDϕc31 | 0.02 min-1 | Degradation rate of Dϕc31 | Assumed |
| kToffBxb1 | 100 nM-2min-1 | Rate of flipping of Ton,i to ToffBxb1 | Assumed |
| k-ToffBxb1 | 100 nM-2min-1 | Rate of flipping of ToffBxb1 to Ton,f | Assumed |
| kToffϕc31 | 100 nM-2min-1 | Rate of flipping of Ton,i to Toffϕc31 | Assumed |
| k-Toffϕc31 | 100 nM-2min-1 | Rate of flipping of Toffϕc31 to Ton,f | Assumed |
| kGFP | 10 min-1 | Production rate of GFP | Assumed |
| kLuxI | 10 min-1 | Production rate of LuxI | Assumed |
| kLasI | 10 min-1 | Production rate of LasI | Assumed |
| dGFP | 0.0049 min-1 | Degradation rate of GFP | Estimated |
| dLuxI | 0.0167 min-1 | Degradation rate of LuxI | Weber |
| dLasI | 0.0167 min-1 | Degradation rate of LasI | Weber |
| kLux-AHL | 0.04 min-1 | Production rate of Lux-AHL (30C6HSL) from the LuxI | Weber |
| kLas-AHL | 0.04 min-1 | Production rate of Las-AHL (30C12HSL) from the LasI | Weber |
