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Team:Bielefeld-CeBiTec/Notebook/StrainsAndConstructs
From 2014.igem.org
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Revision as of 18:29, 11 October 2014
Strains
| Name | Properties / Genotype | BioBrick / Reference |
|---|---|---|
| General | ||
| Escherichia coli KRX | [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase | Promega |
| E. coli DH5α | F-φ80lacZ∆M15∆(lacZYA-argF) U169recA1endA1hsdR17(rk-, mk+)gal-phoAsupE44λ-thi-1gyrA96relA1 | invitrogen |
| Module 1 | ||
| E.coli KRX ΔdcuB::oprF | [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, ΔdcuB::oprF | BBa_K1172507 |
| ΔdcuB | ||
| ΔfrdA | ||
| Actinobacillus succinogenes | ||
| Geobacter sulfurreducens | ||
| Module 2 | ||
| E.coli BL21 | NEB | |
| Module 3 | ||
| JW1228-1 | ΔadhE748::kan | Baba et al., 2006 |
| Biosafety | ||
| E. coli KRX Δalr | [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, Δalr | |
| E. coli KRX ΔdadX | [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, ΔdadX | |
| E. coli KRX Δalr ΔdadX | [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, ΔdadX, Δalr | |
| E. coli DH5α Δalr | F-φ80lacZ∆M15∆(lacZYA-argF) U169recA1endA1hsdR17(rk-, mk+)gal-phoAsupE44λ-thi-1gyrA96relA1 Δalr | |
| E. coli DH5α ΔdadX | F-φ80lacZ∆M15∆(lacZYA-argF) U169recA1endA1hsdR17(rk-, mk+)gal-phoAsupE44λ-thi-1gyrA96relA1 ΔdadX | |
| E. coli DH5α Δalr ΔdadX | F-φ80lacZ∆M15∆(lacZYA-argF) U169recA1endA1hsdR17(rk-, mk+)gal-phoAsupE44λ-thi-1gyrA96relA1 Δalr ΔdadX | |
References
-
Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11
Constructs
| Name | Properties | BioBrick / Reference |
|---|---|---|
| General | ||
| _ | ||
| Module 1 | ||
| _ | ||
| Module 2 | ||
| _ | ||
| Module 3 | ||
| --- | ||
| Biosafety | ||
| --- | ||
References
-
paper
