Team:Bielefeld-CeBiTec/Notebook/StrainsAndConstructs

From 2014.igem.org

(Difference between revisions)
(dh5alpha mutants added)
(table splitted)
Line 31: Line 31:
-
<h1> Strains and Constructs </h1>
+
<h1> Strains </h1>
<div class="element">
<div class="element">
Line 155: Line 155:
  <div id="text">
  <div id="text">
Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11  
Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11  
 +
</div>
 +
</div>
 +
</li>
 +
 +
 +
</ul>
 +
</div>
 +
</div>
 +
 +
 +
 +
<h1> Constructs </h1>
 +
 +
<div class="element">
 +
<table width="100%" cellspacing="10" align="center" style="background-color:transparent">
 +
<tr>
 +
  <td style="font-family:Georgia"><div class="element" style="margin:0px; text-align:center"><a href="#General"><img src="https://static.igem.org/mediawiki/2014/3/35/Bielefeld_CeBiTec_2014-08-25_ecoliGeneral.png"  width="150px" height="150px" align="center"><br>General</a></div></td>
 +
  <td style="font-family:Georgia"><div class="element" style="margin:0px; text-align:center"><a href="#Module1"><img src="https://static.igem.org/mediawiki/2014/3/35/Bielefeld_CeBiTec_2014-08-14_ecoliM1.jpg"  width="150px" height="150px" align="center"><br>Module 1</div></a></td>
 +
  <td style="font-family:Georgia"><div class="element" style="margin:0px; text-align:center"><a href="#Module2"><img src="https://static.igem.org/mediawiki/2014/6/69/Bielefeld_CeBiTec_2014-10-06_ecoliM2.jpg" width="150px" height="150px" align="center"><br>Module 2</div></a></td>
 +
  <td style="font-family:Georgia"><div class="element" style="margin:0px; text-align:center"><a href="#Module3"><img src="https://static.igem.org/mediawiki/2014/d/d7/Bielefeld_CeBiTec_2014-08-14_ecoliM3.jpg" width="150px" height="150px" align="center"><br>Module 3</div></a></td>
 +
  <td style="font-family:Georgia"><div class="element" style="margin:0px; text-align:center"><a href="#Biosafety"><img src="https://static.igem.org/mediawiki/2014/8/8a/Bielefeld_CeBiTec_2014-08-26_ecoliBiosafety.png"  width="150px" height="150px" align="center"><br>Biosafety</div></a></td>
 +
</tr>
 +
</table>
 +
</div>
 +
<br>
 +
 +
<div class="element" style="margin:10px; padding:10px; font-size:15px">
 +
<table width="100%" border="1" cellpadding="5" style="background-color:transparent">
 +
<tr width="100%" bgcolor="#006b60">
 +
  <th width="30%" align="center">Name</th>
 +
  <th width="40%" align="center">Properties</th>
 +
  <th width="30%" align="center">BioBrick / Reference</th>
 +
</tr>
 +
 +
<tr bgcolor="#3fb498">
 +
  <th colspan="3" align="left" id="General">General</th>
 +
</tr>
 +
<tr>
 +
<td width="30%" id="">_</td>
 +
<td width="40%"></td>
 +
<td width="30%"></td>
 +
</tr>
 +
 +
 +
<tr bgcolor="#3fb498">
 +
  <th colspan="3" align="left" id="Module1">Module 1</th>
 +
</tr>
 +
<tr>
 +
<td width="30%" id="">_</td>
 +
<td width="40%"></td>
 +
<td width="30%"></td>
 +
</tr>
 +
 +
<tr bgcolor="#3fb498">
 +
  <th colspan="3" align="left" id="Module2">Module 2</th>
 +
</tr>
 +
<tr>
 +
<td width="30%" id="">_</td>
 +
<td width="40%"></td>
 +
<td width="30%"></td>
 +
</tr>
 +
<tr bgcolor="#3fb498">
 +
  <th colspan="3" align="left" id="Module3">Module 3</th>
 +
</tr>
 +
 +
<tr>
 +
<td width="30%" id=""> --- </td>
 +
<td width="40%"></td>
 +
<td width="30%"></td>
 +
</tr>
 +
 +
<tr bgcolor="#3fb498">
 +
  <th colspan="3" align="left" id="Biosafety">Biosafety</th>
 +
</tr>
 +
<tr>
 +
<td width="30%" id=""> --- </td>
 +
<td width="40%"></td>
 +
<td width="30%"></td>
 +
</tr>
 +
 +
 +
 +
</table>
 +
 +
 +
<div class="element">
 +
  <div id="text">
 +
  <h6>References</h6>
 +
<ul>
 +
 +
<li id="baba2006">
 +
<div class="element" style="margin_10px 10px 10px 10px; padding:10px 10px 10px 10px">
 +
<div id="text">
 +
paper
</div>
</div>
</div>
</div>

Revision as of 18:08, 11 October 2014


Strains


General

Module 1

Module 2

Module 3

Biosafety

Name Properties / Genotype BioBrick / Reference
General
_
Escherichia coli KRX [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase Promega
E. coli DH5α F-φ80lacZ∆M15∆(lacZYA-argF) U169recA1endA1hsdR17(rk-, mk+)gal-phoAsupE44λ-thi-1gyrA96relA1 invitrogen
Module 1
_
Module 2
_
Module 3
JW1228-1 ΔadhE748::kan Baba et al., 2006
Biosafety
E. coli KRX Δalr [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, Δalr
E. coli KRX ΔdadX [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, ΔdadX
E. coli KRX Δalr ΔdadX [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, ΔdadX, Δalr
E. coli DH5α Δalr F-φ80lacZ∆M15∆(lacZYA-argF) U169recA1endA1hsdR17(rk-, mk+)gal-phoAsupE44λ-thi-1gyrA96relA1 Δalr
E. coli DH5α ΔdadX F-φ80lacZ∆M15∆(lacZYA-argF) U169recA1endA1hsdR17(rk-, mk+)gal-phoAsupE44λ-thi-1gyrA96relA1 ΔdadX
E. coli DH5α Δalr ΔdadX F-φ80lacZ∆M15∆(lacZYA-argF) U169recA1endA1hsdR17(rk-, mk+)gal-phoAsupE44λ-thi-1gyrA96relA1 Δalr ΔdadX
References
  • Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11

Constructs


General

Module 1

Module 2

Module 3

Biosafety

Name Properties BioBrick / Reference
General
_
Module 1
_
Module 2
_
Module 3
---
Biosafety
---
References
  • paper

Retrieved from "http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/StrainsAndConstructs"