Team:Aachen/OD/F device
From 2014.igem.org
(→Getting your Device) |
(→Development) |
||
Line 20: | Line 20: | ||
== Development == | == Development == | ||
- | Developing the OD/F device has been an interesting task. On the one hand, this device has been developed mainly by the computer guys. On the other hand, the biologists | + | Developing the OD/F device has been an interesting task. On the one hand, this device has been developed mainly by the computer guys. On the other hand, we got assistance from the biologists suffering from color-blindness, yet eager to help selecting the best color filters for the LEDs. For the next year, you really have to select carefully who's going to help with which task! |
+ | |||
+ | The essential part of this deivce is the cuvette holder and has been developed in an incremental development approach. | ||
+ | First, 3 ml have been consideres, and an optimal height of 1.5 cm from ground has been determined as optimal. | ||
+ | Indeed, this works quite well for the large cuvettes, but unfortunately delivers random<html><sup><a href="#fn1" id="ref1">1</a></sup></html> results on semi-micro cuvettes. | ||
+ | We could determine that the cause of this problem originates from refractions on the sample surface in the cuvette, as this coincedences with the measurement height. | ||
Being naive, the computer guys first attached the sensor to the couvette holder at approximately 1.5cm, which was perfectly suited for 3ml cuvettes. However, those are not widely used anymore, and we migrated to 1.6ml cuvettes. As it turns out, if the sensor sits at 1.5cm, it just hits the position where the cuvette enlarges again - and most importantly, where the edge of the sample solution is. | Being naive, the computer guys first attached the sensor to the couvette holder at approximately 1.5cm, which was perfectly suited for 3ml cuvettes. However, those are not widely used anymore, and we migrated to 1.6ml cuvettes. As it turns out, if the sensor sits at 1.5cm, it just hits the position where the cuvette enlarges again - and most importantly, where the edge of the sample solution is. | ||
Line 47: | Line 52: | ||
Finally, using the dark greenish Twickenham Green filter only little amounts of sub-500nm-light gets through, reducing any bias from this significantly. Unfortunately the transmission rate of this filter is still quite bad at 20% for the target emission wavelength. | Finally, using the dark greenish Twickenham Green filter only little amounts of sub-500nm-light gets through, reducing any bias from this significantly. Unfortunately the transmission rate of this filter is still quite bad at 20% for the target emission wavelength. | ||
Also for the OD measurement we had similar problems. The solution to this problem is presented in the F device section. | Also for the OD measurement we had similar problems. The solution to this problem is presented in the F device section. | ||
+ | |||
+ | <html> | ||
+ | <sup><span class="anchor" id="fn1"></span>1. Quite a good random number generator from a computer-scientific perspective!<a href="#ref1" title="">↩</a></sup> | ||
+ | </html> | ||
== Combined Device == | == Combined Device == |
Revision as of 11:05, 11 October 2014
|
|
|
|