Team:GeorgiaTech

From 2014.igem.org

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     <h2 class="onBlack">How does dissolved methane endanger our water?</h2>
     <h2 class="onBlack">How does dissolved methane endanger our water?</h2>
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Look at this running stream and imagine how it would appear when contaminated with methane gas. If you pictured it as a flowing stream of seltzer water, then you're not far off. Read our <strong>project description</strong> below to learn about our ideas to organically remove methane from water and keep it out of the atmosphere.
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Look at this running stream and imagine how it would appear when contaminated with methane gas. If you pictured it as a flowing stream of seltzer water, then you're not far off.
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The growing demand for natural rubber causes deforestation of the rainforest and occupation of arable lands, due to the establishment of new plantations. If producing rubber by bacteria succeeds, production of natural rubber will not be limited to the regions where the rubber tree can grow. Rather, rubber can be produced even in barren lands.  
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The demand for natural gas to fuel growing industries has led to the development and increased use of fracking techniques to extract methane and other natural gases from shale outcroppings deep in the Earth's crust. These outcroppings often lie underneath freshwater aquifers, and when disturbed can release gases into the water supply posing health concerns for water well owners.  
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Our project aims to enable a common bacteria to produce natural rubber while grown under controlled conditions.
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Our project aims to enable E. coli to produce soluble methane monooxygenase (sMMO) a protein capable of converting methane into less volatile methanol, which can then be treated by further remediation pathways.
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Natural rubber is composed of molecules consisting of the substance IPP linked together like a chain. The common bacteria that we use (E. coli) already possesses the ability to produce the IPP, but it lacks the enzyme to connect the IPP links together into a chain.
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We introduced the enzyme that the rubber tree has for connecting the links into the bacteria. Furthermore, we introduced genes that allow the bacteria further production of the IPP links.  
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Revision as of 18:09, 20 June 2014

How does dissolved methane endanger our water?

Look at this running stream and imagine how it would appear when contaminated with methane gas. If you pictured it as a flowing stream of seltzer water, then you're not far off.

The demand for natural gas to fuel growing industries has led to the development and increased use of fracking techniques to extract methane and other natural gases from shale outcroppings deep in the Earth's crust. These outcroppings often lie underneath freshwater aquifers, and when disturbed can release gases into the water supply posing health concerns for water well owners.

Our project aims to enable E. coli to produce soluble methane monooxygenase (sMMO) a protein capable of converting methane into less volatile methanol, which can then be treated by further remediation pathways.

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