Team:GeorgiaTech/Parts

From 2014.igem.org

Biobricks and Devices

NameTypeDescriptionLength
BBa_K1539001PartCoding sequence has a high efficiency promoter followed by a high efficiency RBS to express fluorescent protein mCherry.814 bp
BBa_K1539002PartCoding sequence has a low efficiency promoter followed by a low efficiency RBS to express fluorescent protein mCherry.814 bp
BBa_K1539003PartThis coding sequence is for the beta subunit for Protein A (composed of three subunits) of the multi-complex enzyme soluble methane monooxyegnase (sMMO). The gene has been codon optimized for E. coli.1170 bp
BBa_K1539005PartThis coding sequence is for the gamma subunit for Protein A (composed of three subunits) of the multi-complex enzyme soluble methane monooxyegnase (sMMO). The gene has been codon optimized for E. coli.513 bp
BBa_K1539008PartThis coding sequence is for the gene sMMO B, encoding Protein B of the multi-complex enzyme soluble methane monooxyegnase (sMMO). The gene has been codon optimized for E. coli.426 bp
BBa_K1539013PartThis coding sequence is for the gene sMMO C, encoding Protein C of the multi-complex enzyme soluble methane monooxyegnase (sMMO). The gene has been codon optimized for E. coli.1047 bp
BBa_K1539021DeviceThis primer is used to insert a high efficiency RBS to transcribe genes starting with ATG in E. coli.46 bp
BBa_K1539034DeviceThis primer is used to insert a low efficiency RBS to transcribe genes starting with ATG in E. coli.46 bp
BBa_K1539055DeviceThis primer is used to insert a high efficiency promoter to translate E. coli. genes. Must be used after insertion of RBS primer. Use Georgia Tech iGEM RBS primers (BBa_K1539021 or BBa_K1539034) or an RBS primer with a similar design.58 bp
BBa_K1539089DeviceThis primer is used to insert a low efficiency promoter to translate E. coli. genes. Must be used after insertion of RBS primer. Use Georgia Tech iGEM RBS primers (BBa_K1539021 or BBa_K1539034) or an RBS primer with a similar design.58 bp