Team:Hong Kong HKUST/wetlab/protocols

From 2014.igem.org

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<a href="https://static.igem.org/mediawiki/2014/7/7c/12Mutagenesis%28new%29.pdf">
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<a href="https://static.igem.org/mediawiki/2014/8/84/BD_FACSAria_III_User_Guide_%281%29.pdf">
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<h4> Mutagenesis</h4>
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<h4> BD FACSARiaIII User Guide</h4>
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<h4> Western Blot </h4>
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<h4> Dephosphorylation </h4>
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<a href="https://static.igem.org/mediawiki/2014/2/25/19._Ethanol_Precipitation.pdf">
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<h4> LB agar and LB plates </h4>
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<h4> Ethanol Precipitation </h4>
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<a href="https://static.igem.org/mediawiki/2014/e/e9/10._Ligation.pdf">
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<a href="https://static.igem.org/mediawiki/2014/6/64/Gibson_assembly_master_mix-_assembly.pdf">
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<h4> Gibson Assembly </h4>
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<a href="https://static.igem.org/mediawiki/2014/e/e9/10._Ligation.pdf">
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<h4> Ligation </h4>
<h4> Ligation </h4>
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<a href="https://static.igem.org/mediawiki/2014/f/f3/Liquid_mediums_and_Agarose_and_Plates.pdf">
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<h4> Liquid Mediums and Agarose Plates </h4>
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<a href="https://static.igem.org/mediawiki/2014/8/8b/M9_Minimal_medium_protocol.pdf">
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<h4> M9 Minimal Medium </h4>
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<a href="https://static.igem.org/mediawiki/2014/2/2a/Mutagenesis_protocol_1_%28multiple_sites%29.pdf">
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<h4> Mutagenesis 1(Multiple Sites)</h4>
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<a href="https://static.igem.org/mediawiki/2014/1/1f/Mutagenesis_protocol_2_%28single_site%29.pdf">
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<h4> Ethanol Precipitation</h4>
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<h4> Mutagenesis 2 (Single Sites) </h4>
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<a href="https://static.igem.org/mediawiki/2014/2/27/Pbad.pdf">
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<a href="https://static.igem.org/mediawiki/2014/4/42/Mutagenesis_protocol_3_%28single_site%29.pdf">
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<h4> Pbad Protocols </h4>
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<h4> Mutagenesis 3 (Single Sites) </h4>
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<a href="https://static.igem.org/mediawiki/2014/a/ad/Oligonucleotide_phosoporilation_and_annealing.pdf">
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<h4> Gibson Assembly </h4>
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<h4> Oligonucleotide Phosphorylation and Annealing </h4>
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<a href="https://static.igem.org/mediawiki/2014/d/d0/Protocol_for_a_routine_Taq_PCR.pdf">
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<h4> PCR (routine <i>Taq</i>)</h4>
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<h4> PCR (Phusion) </h4>
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<a href="https://static.igem.org/mediawiki/2014/c/c5/Protocol_for_Vent_PCR.pdf">
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<h4> PCR (Vent) </h4>
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<a href="https://static.igem.org/mediawiki/2014/7/76/RPU_Measurement_Method_.pdf">
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<h4> RPU Measurement </h4>
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<a href="https://static.igem.org/mediawiki/2014/4/4d/The_Inoue_Method_for_Preparation_and_Transformation_of_Competent_E.pdf">
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<h4> Polymerase Chain Reaction (PCR)</h4>
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<h4> The Inoue Method for Preparation and Transformation of Competent E. Coli: "Ultra-Competent" Cells</h4>
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Revision as of 15:36, 10 October 2014



Protocols

We have a variety of protocols that we followed for working on our project. The step-by-step procedures of each technique can be found here!

Home

Pneumosensor

Riboregulator

Human Practice

Team

WetLab

Achievement

Retrieved from "http://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/protocols"