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Team:Hong Kong HKUST/data
From 2014.igem.org
(Difference between revisions)
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<div class= "content_area_two_row"> | <div class= "content_area_two_row"> | ||
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How our system works:<br> | How our system works:<br> | ||
[Diagram with Biobrick Code] | [Diagram with Biobrick Code] | ||
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<b>Data For Our Favorite New Parts:</b> | <b>Data For Our Favorite New Parts:</b> | ||
| - | <ul | + | <ul> |
<li> <b>SigmaX, <a href= "http://parts.igem.org/Part:BBa_K1379004"> BBa_K1379004</a></b>: gene sequence of competence protein comX which produces σ<sup>x</sup> factor. σ<sup>x</sup> factor induces promoter containing combox sequence (P<sub>celA</sub> and P<sub>helicase</sub>). | <li> <b>SigmaX, <a href= "http://parts.igem.org/Part:BBa_K1379004"> BBa_K1379004</a></b>: gene sequence of competence protein comX which produces σ<sup>x</sup> factor. σ<sup>x</sup> factor induces promoter containing combox sequence (P<sub>celA</sub> and P<sub>helicase</sub>). | ||
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<b>Data for Pre-existing Parts:</b> | <b>Data for Pre-existing Parts:</b> | ||
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| - | <li><b>Strong promoter, strong RBS combination, <a href= "http://parts.igem.org/Part:BBa_K880005"> BBa_K880005 </a></b>(Team Michigan iGEM, 2012): combination of strong constitutive promoter (<a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_J23100"> BBa_J23100</a>) and strong RBS (<a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034">BBa_B0034</a>) was used as a backbone for a very high level of expression of ComX protein (σ<sup>x</sup> factor). | + | <li><b>Strong promoter, strong RBS combination, <a href= "http://parts.igem.org/Part:BBa_K880005"> BBa_K880005 </a></b>(Team Michigan iGEM, 2012): combination of strong constitutive promoter (<a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_J23100"> BBa_J23100</a>) and strong RBS (<a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034"> BBa_B0034 </a>) was used as a backbone for a very high level of expression of ComX protein (σ<sup>x</sup> factor). |
| - | <li><b>GFP generator</b>, <a href= "http://parts.igem.org/Part:BBa_E0240"> BBa_E0240</a></b>(Antiquity Boston University iGEM, 2004): green fluorescent protein reporter (<a href= "http://parts.igem.org/Part:BBa_E0240">BBa_E0040</a>) with RBS (<a href= "http://parts.igem.org/Part:BBa_B0032"> BBa_B0032</a>) and double terminator (<a href= "http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>) was used as a reporter for characterization of P<sub>celA</sub> and P<sub>helicase</sub> promoters. | + | <li><b>GFP generator</b>, <a href= "http://parts.igem.org/Part:BBa_E0240"> BBa_E0240 </a></b>(Antiquity Boston University iGEM, 2004): green fluorescent protein reporter (<a href= "http://parts.igem.org/Part:BBa_E0240"> BBa_E0040 </a>) with RBS (<a href= "http://parts.igem.org/Part:BBa_B0032"> BBa_B0032</a>) and double terminator (<a href= "http://parts.igem.org/Part:BBa_B0015"> BBa_B0015</a>) was used as a reporter for characterization of P<sub>celA</sub> and P<sub>helicase</sub> promoters. |
</ul> | </ul> | ||
<br> | <br> | ||
<br> | <br> | ||
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<b>We Also Built and Characterized the Following Parts</b> | <b>We Also Built and Characterized the Following Parts</b> | ||
| - | <ul | + | <ul> |
<li> <b>SigmaX Generator, <a href="http://parts.igem.org/Part:BBa_K1379006"> BBa_K1379006</a></b>: <i>comX</i> gene sequence was assembled with constitutive promoter, RBS and double terminator so ComX protein is produced constitutively. | <li> <b>SigmaX Generator, <a href="http://parts.igem.org/Part:BBa_K1379006"> BBa_K1379006</a></b>: <i>comX</i> gene sequence was assembled with constitutive promoter, RBS and double terminator so ComX protein is produced constitutively. | ||
<li> <b> P<sub>celA</sub>-GFP Generator, <a href="http://parts.igem.org/Part:BBa_K1379002"> BBa_K1379002</a></b>: σ<sup>x</sup>-inducible P<sub>celA</sub> promoter was assembled with GFP generator. | <li> <b> P<sub>celA</sub>-GFP Generator, <a href="http://parts.igem.org/Part:BBa_K1379002"> BBa_K1379002</a></b>: σ<sup>x</sup>-inducible P<sub>celA</sub> promoter was assembled with GFP generator. | ||
| - | <li> <b>P<sub>helicase</sub>-GFP Generator, <a href= "http://parts.igem.org/Part:BBa_K1379003">BBa_K1379003</a></b>: σ<sup>x</sup>-inducible P<sub>helicase</sub> promoter was assembled with GFP generator. | + | <li> <b>P<sub>helicase</sub>-GFP Generator, <a href= "http://parts.igem.org/Part:BBa_K1379003"> BBa_K1379003</a></b>: σ<sup>x</sup>-inducible P<sub>helicase</sub> promoter was assembled with GFP generator. |
| - | <li> <b>SigmaX Generator, P<sub>celA</sub>-GFP Generator, <a href= "http://parts.igem.org/Part:BBa_K1379005">BBa_K1379005</a></b>: σ<sup>x</sup>-generator was assembled with P<sub>celA</sub>-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed. | + | <li> <b>SigmaX Generator, P<sub>celA</sub>-GFP Generator, <a href= "http://parts.igem.org/Part:BBa_K1379005"> BBa_K1379005</a></b>: σ<sup>x</sup>-generator was assembled with P<sub>celA</sub>-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed. |
| - | <li> <b>SigmaX Generator, P<sub>helicase</suB>-GFP Generator, <a href="http://parts.igem.org/Part:BBa_K1379007">BBa_K1379007</a></b>: σ<sup>x</sup>-generator was assembled with P<sub>helicase</sub>-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed. | + | <li> <b>SigmaX Generator, P<sub>helicase</suB>-GFP Generator, <a href="http://parts.igem.org/Part:BBa_K1379007"> BBa_K1379007</a></b>: σ<sup>x</sup>-generator was assembled with P<sub>helicase</sub>-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed. |
</ul> | </ul> | ||
</p> | </p> | ||
Revision as of 13:21, 10 October 2014
Pneumosensor Data Page
This page shows all the parts we have made or used in our whole project. Click on the links below to see our constructs on the Registry of Standard Biological Parts in detail. Click here to access our result page for an overview of our achievements with those parts.
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How our system works:
Data for Pre-existing Parts:
We Also Built and Characterized the Following Parts
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