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iGEM TEAM HEIDELBERG 2014

THE RING
OF FIRE

Welcome to the proteins of tomorrow.
This is the iGEM team Heidelberg‘s wiki page.

Click here to view our abstract.
Scroll down to explore our project.

PLACEHOLDER

Nature has made many curious inventions. One of these are circular proteins, which are conventional peptides that neither have a beginning, nor an ending

Apart from other features, these proteins are extremely stable against high temperatures, pH and proteases.

We seeked to apply this principle of circularization in Synthetic Biology and create a way of rendering any protein heatstable.

Let us take you to the next level of bioengineering ...

USING THE Mechanism OF SPLIT-INTEINS

Intein splicing is a natural process that excises one part of a protein and leaves the remaining parts irreersibly attached.

When attaching split inteins to the ends of a normal protein, the splicing reaction connects the beginning to the ending and forms a circular protein.

Placeholder

CIRCULARIZATION

OLIGOMERIZATION

FUSION

ON/OFF

PURIFICATION

... and show you the WORLD of
post-translational MODIFCATION

The iGEM Team Heidelberg has developed an intein toolbox for the iGEM community to easily modify your protein in a standarized method.

Our toolbox contains several tools which are supergeil. Here you can find out more about our Toolbox.

In addition to that all tools are inducible by light. Using the LOV system we built a solid method for regulation of the intein trans-splicing reaction our toolbox consiting on. Click here to get more informations about Induction.

circular heat-stable
DNMT1

Wouldn´t it be great to amplify DNA in a normal PCR maintaining the epigenetic information coded in methylation patterns?

The problem: DNMT I, an enzyme which is responsible for the establishment and maintenance of the individual methylation pattern of different cell types, is not heat stable. For iGEM 2014 we therefore create a PCR 2.0 with heat-stable DNMT I by circularization.

PCR 2.0

circular heat-stable
Xylanase

Xylanase is an important enzyme for the pulp and paper industry.

Bla bla

In future Xylanase could be used for the production of biofuel.

INDUSTRY

LINK it!

Could every protein becomes heat stable by circularization, even if it´s the most complex of all?

Circularization is a narrow path between gaining heat-stability and loosing function due to deformation. We developed a linker software, which predict the perfect linker depending on the folding structure of every protein.

In an extensive linker screening our software was improved and calibrated using the lambda phage lysozyme.

CALCULATE it!

After calculating eleven days and the breakdown of both computational and mental power we decided to spread the modeling of the linkers.

The iGEM Team Heidelberg developed iGEM@home, a software to divide extensive computing task into many packages and to distribute them to many computers. Now over 1,000 volunteers are calculating for us when their are idle.

As a new tool for the iGEM community this system enables every student team to archieve their modeling without access to big server farms.

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