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Team:NCTU Formosa/Notebook/test3
From 2014.igem.org
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PCR: [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2) | PCR: [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2) | ||
Cultivation of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2) | Cultivation of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2) | ||
| - | Electrophoresis of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3]Kr and [B0032+BFP*3] Kr plasmid (G2) | + | Electrophoresis of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3]Kr and [B0032+BFP*3] Kr plasmid (G2) </p> |
| - | </p><p> | + | <p> |
6/3 | 6/3 | ||
PCR: [T7 promoter*5] Ar Kr, [B0032+BFP*5] Kr , [J61048+J61048*5] Cr and [T7polymerase+J61048*5] Kr (G2) | PCR: [T7 promoter*5] Ar Kr, [B0032+BFP*5] Kr , [J61048+J61048*5] Cr and [T7polymerase+J61048*5] Kr (G2) | ||
Electrophoresis of PCR products (G2) | Electrophoresis of PCR products (G2) | ||
| - | Cultivation of [B0032+BFP*10] Kr, [J61048+J61048*10] Cr and [T7polymerase+J61048*5] Kr (G2) | + | Cultivation of [B0032+BFP*10] Kr, [J61048+J61048*10] Cr and [T7polymerase+J61048*5] Kr (G2)</p> |
| - | </p><p> | + | <p> |
6/5 | 6/5 | ||
Transform of ligation product [K592100+J61048+PSBIK3] Kr (G1) | Transform of ligation product [K592100+J61048+PSBIK3] Kr (G1) | ||
Ligation of [BFP+B0032] Kr and [T7polymerase+J61048] Kr (G2) | Ligation of [BFP+B0032] Kr and [T7polymerase+J61048] Kr (G2) | ||
| - | Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3) | + | Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)</p> |
| - | </p><p> | + | <p> |
6/6 | 6/6 | ||
Mini-prep of [J61048+J61048*3] Cr and [T7 promoter*3]Ar Kr (G2) | Mini-prep of [J61048+J61048*3] Cr and [T7 promoter*3]Ar Kr (G2) | ||
Digestion of [J61048+J61048*3] Cr (XP) and [T7 promoter*3]Ar Kr(ES) (G2) | Digestion of [J61048+J61048*3] Cr (XP) and [T7 promoter*3]Ar Kr(ES) (G2) | ||
Transform of [B0032+BFP] Kr and [T7polymerase+J61048] Kr (G2) | Transform of [B0032+BFP] Kr and [T7polymerase+J61048] Kr (G2) | ||
| - | Transform of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3) | + | Transform of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)</p> |
| - | </p><p> | + | <p> |
6/7 | 6/7 | ||
PCR: [PSB1K3*6] Kr, [K592100+J61048+PSB1K3*10] Kr,[J23101*8] Ar ,[B0032*8] Ar (G1) | PCR: [PSB1K3*6] Kr, [K592100+J61048+PSB1K3*10] Kr,[J23101*8] Ar ,[B0032*8] Ar (G1) | ||
| - | PCR: [mGFP+B0015+PSB1K3*6] Kr and [mGFP+J61048+PSB1K3*6] Kr (G3) | + | PCR: [mGFP+B0015+PSB1K3*6] Kr and [mGFP+J61048+PSB1K3*6] Kr (G3)</p> |
| - | </p | + | |
| - | + | ||
| + | <br></div> | ||
<div class="single note"> | <div class="single note"> | ||
<p> | <p> | ||
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PCR: [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2) | PCR: [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2) | ||
Cultivation of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2) | Cultivation of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2) | ||
| - | Electrophoresis of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2) | + | Electrophoresis of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2)</p> |
| - | </p> | + | |
<p> | <p> | ||
6/9 | 6/9 | ||
Transform of [B0023*2 ] Ar ,[J23101*2] Ar, [K592100+J61048*2] Kr and [PSB1K3*2] Kr (G1) | Transform of [B0023*2 ] Ar ,[J23101*2] Ar, [K592100+J61048*2] Kr and [PSB1K3*2] Kr (G1) | ||
Electrophoresis of plasmid and digestion product of [RBS+BFP]Kr (ES),[T7 polymerase+J61048]Kr(XP),[T7 promoter]Kr Cr (ES),[J61048+J61048]Cr(XP) (G2) | Electrophoresis of plasmid and digestion product of [RBS+BFP]Kr (ES),[T7 polymerase+J61048]Kr(XP),[T7 promoter]Kr Cr (ES),[J61048+J61048]Cr(XP) (G2) | ||
| - | Mini-prep of the plasmid and then digested the plasmid:[T7 polymerase+J61048]Kr(XP),[RBS+BFP]Kr(ES) (G2) | + | Mini-prep of the plasmid and then digested the plasmid:[T7 polymerase+J61048]Kr(XP),[RBS+BFP]Kr(ES) (G2)</p> |
| - | </p> | + | |
<p> | <p> | ||
6/10 | 6/10 | ||
| - | Electrophoresis of plasmid and digestion product of [K592100+J61048*2] Kr (XP), [PSB1K3*2] Kr (EP),[J23101*2] Ar (SP) and [B0023*2 ] Ar (SP) (G1) | + | Electrophoresis of plasmid and digestion product of [K592100+J61048*2] Kr (XP), [PSB1K3*2] Kr (EP),[J23101*2] Ar (SP) and [B0023*2 ] Ar (SP) (G1)</p> |
| - | </p> | + | |
<p> | <p> | ||
6/11 | 6/11 | ||
| - | Digestion of [mGFP] Ar (ES), [J61048] Ar (SP) ,[B0015] Ar (SP) (G3) | + | Digestion of [mGFP] Ar (ES), [J61048] Ar (SP) ,[B0015] Ar (SP) (G3)</p> |
| - | </p> | + | |
<p> | <p> | ||
6/12 | 6/12 | ||
Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3) | Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3) | ||
| - | PCR: [Plac+B0032+PSB1K3*12] Kr (G3) | + | PCR: [Plac+B0032+PSB1K3*12] Kr (G3)</p> |
| - | </p><p> | + | <p> |
6/13 | 6/13 | ||
Transform of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3) | Transform of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3) | ||
| - | Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3) | + | Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3)</p> |
| - | </p> | + | |
<p> | <p> | ||
6/14 | 6/14 | ||
Mini-prep of [Plac+B0032+PSB1K3*2] Kr (G3) | Mini-prep of [Plac+B0032+PSB1K3*2] Kr (G3) | ||
Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3) | Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3) | ||
| - | PCR: [mGFP+B0015+PSB1K3*6] Kr | + | PCR: [mGFP+B0015+PSB1K3*6] Kr </p> |
| - | </p> | + | <br></div> |
| - | </div> | + | |
<div class="single note"> | <div class="single note"> | ||
<p> | <p> | ||
6/15 | 6/15 | ||
Mini-prep [Plac+B0032+PSB1K3*4] Kr (G3) | Mini-prep [Plac+B0032+PSB1K3*4] Kr (G3) | ||
| - | Digestion of [Plac+B0032+PSB1K3*4] Kr (ES) (G3) | + | Digestion of [Plac+B0032+PSB1K3*4] Kr (ES) (G3)</p> |
| - | </p> | + | |
<br></div> | <br></div> | ||
| - | |||
<div class="single note"> | <div class="single note"> | ||
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Ligation of [B0032+K592100+J61048] Ar (G1) | Ligation of [B0032+K592100+J61048] Ar (G1) | ||
Electrophoresis of plasmid and digestion product of [K592100+J61048] Kr (XP), [J23101] Ar (SP) and [B0032] Ar (SP) (G1) | Electrophoresis of plasmid and digestion product of [K592100+J61048] Kr (XP), [J23101] Ar (SP) and [B0032] Ar (SP) (G1) | ||
| - | Digestion of [T7 polymerase +J61048]Kr(XP),[J61048+J61048]Cr(XP) (G2) | + | Digestion of [T7 polymerase +J61048]Kr(XP),[J61048+J61048]Cr(XP) (G2)</p> |
| - | </p> | + | |
<p> | <p> | ||
6/27 | 6/27 | ||
Digestion of [J61048+J61048] Cr (XP) (G2) | Digestion of [J61048+J61048] Cr (XP) (G2) | ||
| - | Electrophoresis of [RBS+BFP]kr(ES)(mini).[T7polymerase](ES)(mini).[T7 promoter](mini) | + | Electrophoresis of [RBS+BFP]kr(ES)(mini).[T7polymerase](ES)(mini).[T7 promoter](mini)</p> |
| - | </p> | + | |
<p> | <p> | ||
6/28 | 6/28 | ||
| - | Transformation of mini:[B0032+BFP+J61048] Cr (G2) | + | Transformation of mini:[B0032+BFP+J61048] Cr (G2)</p> |
| - | </p> | + | |
| - | + | ||
<br></div> | <br></div> | ||
<div class="single note"> | <div class="single note"> | ||
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6/29 | 6/29 | ||
PCR:[B0032+BFP+J61048]Cr then | PCR:[B0032+BFP+J61048]Cr then | ||
| - | Electrophoresis of the PCR products (G2) | + | Electrophoresis of the PCR products (G2)</p> |
| - | </p> | + | |
<br></div> | <br></div> | ||
| + | |||
| + | </div> | ||
| + | <div class="clear"></div> | ||
</div> | </div> | ||
| - | |||
Revision as of 16:17, 9 October 2014
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6/2 PCR: [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2) Cultivation of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2) Electrophoresis of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3]Kr and [B0032+BFP*3] Kr plasmid (G2)
6/3 PCR: [T7 promoter*5] Ar Kr, [B0032+BFP*5] Kr , [J61048+J61048*5] Cr and [T7polymerase+J61048*5] Kr (G2) Electrophoresis of PCR products (G2) Cultivation of [B0032+BFP*10] Kr, [J61048+J61048*10] Cr and [T7polymerase+J61048*5] Kr (G2)
6/5 Transform of ligation product [K592100+J61048+PSBIK3] Kr (G1) Ligation of [BFP+B0032] Kr and [T7polymerase+J61048] Kr (G2) Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)
6/6 Mini-prep of [J61048+J61048*3] Cr and [T7 promoter*3]Ar Kr (G2) Digestion of [J61048+J61048*3] Cr (XP) and [T7 promoter*3]Ar Kr(ES) (G2) Transform of [B0032+BFP] Kr and [T7polymerase+J61048] Kr (G2) Transform of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)
6/7 PCR: [PSB1K3*6] Kr, [K592100+J61048+PSB1K3*10] Kr,[J23101*8] Ar ,[B0032*8] Ar (G1) PCR: [mGFP+B0015+PSB1K3*6] Kr and [mGFP+J61048+PSB1K3*6] Kr (G3)
6/8 Electrophoresis of plasmid and digestion product of [J61048+J61048](XP), [T7 promoter](ES) PCR: [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2) Cultivation of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2) Electrophoresis of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2)
6/9 Transform of [B0023*2 ] Ar ,[J23101*2] Ar, [K592100+J61048*2] Kr and [PSB1K3*2] Kr (G1) Electrophoresis of plasmid and digestion product of [RBS+BFP]Kr (ES),[T7 polymerase+J61048]Kr(XP),[T7 promoter]Kr Cr (ES),[J61048+J61048]Cr(XP) (G2) Mini-prep of the plasmid and then digested the plasmid:[T7 polymerase+J61048]Kr(XP),[RBS+BFP]Kr(ES) (G2)
6/10 Electrophoresis of plasmid and digestion product of [K592100+J61048*2] Kr (XP), [PSB1K3*2] Kr (EP),[J23101*2] Ar (SP) and [B0023*2 ] Ar (SP) (G1)
6/11 Digestion of [mGFP] Ar (ES), [J61048] Ar (SP) ,[B0015] Ar (SP) (G3)
6/12 Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3) PCR: [Plac+B0032+PSB1K3*12] Kr (G3)
6/13 Transform of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3) Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3)
6/14 Mini-prep of [Plac+B0032+PSB1K3*2] Kr (G3) Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3) PCR: [mGFP+B0015+PSB1K3*6] Kr
6/15 Mini-prep [Plac+B0032+PSB1K3*4] Kr (G3) Digestion of [Plac+B0032+PSB1K3*4] Kr (ES) (G3)
6/24 Digestion of [J23101] Ar (ES) and [B0032] Ar (XP) (G1) Ligation of [B0032+K592100+J61048] Ar (G1) Electrophoresis of plasmid and digestion product of [K592100+J61048] Kr (XP), [J23101] Ar (SP) and [B0032] Ar (SP) (G1) Digestion of [T7 polymerase +J61048]Kr(XP),[J61048+J61048]Cr(XP) (G2)
6/27 Digestion of [J61048+J61048] Cr (XP) (G2) Electrophoresis of [RBS+BFP]kr(ES)(mini).[T7polymerase](ES)(mini).[T7 promoter](mini)
6/28 Transformation of mini:[B0032+BFP+J61048] Cr (G2)
6/29 PCR:[B0032+BFP+J61048]Cr then Electrophoresis of the PCR products (G2)
