Vectors Construction

We have successfully constructed 2 backbones, piGEM001 and piGEM002. And we have verified them using digestion (Fig. 1 and Fig. 2) and sequencing.

Then we have successfully constructed 6 monogene expression vectors, from vector piGEM003 to vector piGEM008. And we have verified all of them using digestion and sequcencing. Here we only show the result of vector piGEM005(Fig. 3). You can browse notebook for more results.

In order to enhance the ability of tobacco to metabolize formaldehyde, we have successfully constructed 3 multigenge expression vectors, from vector piGEM009 to vector piGEM011.We have verified all of them using digestion (Fig.4, Fig.5 and Fig.6) and sequcencing.

Plant transformation

Tobacco was transformed essentially using the leaf disk co-cultivation protocol of Horsch.This protocol includes three stages, co-cultivation (Fig. 7), screening cultivation (Fig. 8) and rooting cultivation (Fig.9). We have successfully transformed each vector into babacco