Team:UC Davis/Notebook Electrochemistry

From 2014.igem.org

(Difference between revisions)
Line 97: Line 97:
       </table>
       </table>
<div class="notebookcontent">
<div class="notebookcontent">
-
<p><h1>Week XX</h1></p>
+
<p><h1>Week 1</h1></p>
<p>PLACE CONTENT HERE</P>
<p>PLACE CONTENT HERE</P>
<PLACE ANOTHER PARAGRAPH OF CONTENT HERE</P>
<PLACE ANOTHER PARAGRAPH OF CONTENT HERE</P>
 +
<h3>6/16</h3>
<ul>
<ul>
-
<li>SAMPLE LIST ITEM 1</li>
+
<li>Settled on paper: An improved biosensor for acetaldehyde detection using a bienzymic strategy at poly(neutral red) modified carbon film electrodes by Ghica et Al.</li>
-
<li>SAMPLE LIST ITEM 2</li>
+
<li>Emailed professors at UCD as well as paper authors for advice/consulting regarding electrochemical cell production. Dr. Land for his experience with surface chemistry, Dr. Revzin for his experience with biomaterials and biosensors, Dr. Stroeve for his experience in colloids, surfaces, and biosensors, Dr. Berben for her experience with electrochemistry, and Dr. Brett, Dr. Pauliukaite, and Dr. Ghica (PI’s involved in the paper mentioned previously) for their work in constructing electrochemical cells.
-
<li>SAMPLE LIST ITEM 3</li>
+
</li>
</ul>
</ul>
 +
 +
<h3>6/18</h3>
 +
<ul>
 +
<li>
 +
Wrote up protocol for production from carbon film electrode synthesis to implementation of biosensor in a device. Based on paper mentioned above (An improved biosens…) as well as several papers by the same authors describing synthesis methods for the carbon electrodes. Carbon film electrode papers: Carbon Film Resistors as Electrodes: Voltammetric Properties and Applications in Electrolysis, Characterization of Carbon Film Electrodes for Electroanalysis by Electrochemical Impedance.
 +
</li>
 +
<li>
 +
Confirmed meetings with several professors (Land, Revzin, Berben)
 +
</li>
 +
<li>
 +
Met with Dr. Wang, Olive Oil Expert. She provided several aldehyde samples as well as discussed olive oil progress.
 +
</li>
 +
<li>
 +
Wrote back to paper authors, looking to get in touch with Dr. Leiss at the department of physics at the university of Munich. He provided Ghica et Al. with the carbon film electrodes necessary for biosensor construciton.
 +
</li>
 +
<li>
 +
Underwent general lab safety training with Diane Hoffman (2 hrs)
 +
</li>
 +
</ul>
 +
 +
<h3>6/19</h3>
 +
<ul>
 +
<li>
 +
Met with Dr. Berben, electrochemistry expert.
 +
  <ul>
 +
  <li>
 +
There is a characteristic base capacitative current associated with the particular electrode
 +
  </li>
 +
  <li>
 +
Good source for electrodes: CH Instruments
 +
  </li>
 +
  <li>
 +
Pt counter electrode is a good choice; ensure large enough size. SCE or AgCl electrode would work interchangeably. AgCl is much cheaper, and doesn’t pose a safety hazard.
 +
  </li>
 +
  <li>
 +
Ionic liquids are a good choice for solvent - conducting, solubilizing both non-polar olive oil, as well as polar Phosphate buffer solution. However, they may be expensive, or difficult to obtain. THF or Hexane may be different options to consider, though they may have an impact on protein activity.
 +
  </li>
 +
  <li>
 +
Interference from other species in the olive oil should not be an issue as the redox potential of most organic compounds in the oil will be far above the working potential of the electrode. In any case, run standards to account for possible interference.
 +
  </li>
 +
  </ul>
 +
</li>
 +
<li>
 +
Met with Dr. Revzin, biomaterials expert.
 +
 +
  <ul>
 +
  <li>
 +
Has professional potentiostat that may be of use when we construct electrode
 +
  </li>
 +
  <li>
 +
Willing to help with the troubleshooting process.
 +
  </li>
 +
  <li>
 +
Protocol looks good. Only testing will tell if it works.
 +
  </li>
 +
  </ul>
 +
</li>
 +
Underwent further safety training
 +
<li>
 +
Brainstormed for general presentation.
 +
</li>
 +
</ul>
 +
 +
<h3>6/20</h3>
 +
<ul>
 +
<li>
 +
Made rough parts list spreadsheet for electrochemical device
 +
</li>
 +
<li>
 +
Made rough pitch presentation.
 +
</li>
 +
<li>
 +
Group meeting, presented week’s work and findings
 +
</li>
 +
Met with Dr. Land, Surface Chemistry Expert
 +
<li>
 +
  <ul>
 +
  <li>
 +
XPS for surface bond characterization
 +
  </li>
 +
  <li>
 +
Electron microscopy best bet for estimate of surface rougness and landscape
 +
  </li>
 +
  <li>
 +
Could do Auger Electron Spectroscopy (Native to some EM’s) to determine surface coverage of polymer films etc…
 +
  </li>
 +
  </ul>
 +
</li>
 +
</ul>
 +
</div>
</div>
</div>
</div>

Revision as of 17:43, 8 October 2014

UC Davis iGEM 2014

Protein Engineering

Protein Engineering

Electrochemistry

Electrochemistry

Potentiostat Design

Potentiostat Design
June 16-200
June 23-271
June 30-July 42
July 7-113
July 14-184
July 21-255
July 28-August 16
August 4-87
August 11-158
August 18-239
August 25-3010
September 1-711
September 8-1412
September 15-2113
September 22-2814
September 29-October 515
October 6-1216
October 13-1917
October 20-2618
October 27-1919

Week 1

PLACE CONTENT HERE

6/16

  • Settled on paper: An improved biosensor for acetaldehyde detection using a bienzymic strategy at poly(neutral red) modified carbon film electrodes by Ghica et Al.
  • Emailed professors at UCD as well as paper authors for advice/consulting regarding electrochemical cell production. Dr. Land for his experience with surface chemistry, Dr. Revzin for his experience with biomaterials and biosensors, Dr. Stroeve for his experience in colloids, surfaces, and biosensors, Dr. Berben for her experience with electrochemistry, and Dr. Brett, Dr. Pauliukaite, and Dr. Ghica (PI’s involved in the paper mentioned previously) for their work in constructing electrochemical cells.

6/18

  • Wrote up protocol for production from carbon film electrode synthesis to implementation of biosensor in a device. Based on paper mentioned above (An improved biosens…) as well as several papers by the same authors describing synthesis methods for the carbon electrodes. Carbon film electrode papers: Carbon Film Resistors as Electrodes: Voltammetric Properties and Applications in Electrolysis, Characterization of Carbon Film Electrodes for Electroanalysis by Electrochemical Impedance.
  • Confirmed meetings with several professors (Land, Revzin, Berben)
  • Met with Dr. Wang, Olive Oil Expert. She provided several aldehyde samples as well as discussed olive oil progress.
  • Wrote back to paper authors, looking to get in touch with Dr. Leiss at the department of physics at the university of Munich. He provided Ghica et Al. with the carbon film electrodes necessary for biosensor construciton.
  • Underwent general lab safety training with Diane Hoffman (2 hrs)

6/19

  • Met with Dr. Berben, electrochemistry expert.
    • There is a characteristic base capacitative current associated with the particular electrode
    • Good source for electrodes: CH Instruments
    • Pt counter electrode is a good choice; ensure large enough size. SCE or AgCl electrode would work interchangeably. AgCl is much cheaper, and doesn’t pose a safety hazard.
    • Ionic liquids are a good choice for solvent - conducting, solubilizing both non-polar olive oil, as well as polar Phosphate buffer solution. However, they may be expensive, or difficult to obtain. THF or Hexane may be different options to consider, though they may have an impact on protein activity.
    • Interference from other species in the olive oil should not be an issue as the redox potential of most organic compounds in the oil will be far above the working potential of the electrode. In any case, run standards to account for possible interference.
  • Met with Dr. Revzin, biomaterials expert.
    • Has professional potentiostat that may be of use when we construct electrode
    • Willing to help with the troubleshooting process.
    • Protocol looks good. Only testing will tell if it works.
    • Underwent further safety training
  • Brainstormed for general presentation.

6/20

  • Made rough parts list spreadsheet for electrochemical device
  • Made rough pitch presentation.
  • Group meeting, presented week’s work and findings
    • Met with Dr. Land, Surface Chemistry Expert
    • XPS for surface bond characterization
    • Electron microscopy best bet for estimate of surface rougness and landscape
    • Could do Auger Electron Spectroscopy (Native to some EM’s) to determine surface coverage of polymer films etc…

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