Future Work

Due to time reasons, we have not clone the stomatal regulation gene, AtAHA2, to the expression vector. So our next target would be cloning the AtAHA2 gene and its promoter, GC1, to the expressing vector, transforming that to plants, and testing their abilities of absorbing formaldehyde.

The chloroplast is a pivotal organelle in plant cells and eukaryotic algae to carry out photosynthesis, which provides the primary source of the world’s food. The expression of foreign genes in chloroplasts offers several a dvantages over their expression in the nucleus: high-level expression, transgene stacking in operons and a lack of epigenetic interference allowing stable transgene expression. In addition, transgenic chloroplasts are generally not transmitted through pollen grains because of the cytoplasmic localization. And it meets the requirement of biosafety of iGEM. In the past two decades, great progress in chloroplast engineering has been made. So in the future work, we will tranform the genes into the chloroplast of plants.

By now our project is still in its basic research stage. So we experiments was on the model plant, tobacco. Next time we will try it on some ornamental plants.