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Team:Hong Kong HKUST/wetlab/notebook
From 2014.igem.org
(Difference between revisions)
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<a href="#" class="head">Week 1</a> | <a href="#" class="head">Week 1</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br><b>Aug | + | <br><b>Aug 4</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| - | · <br> | + | · Digestion of comX-myc on EcoRI-HF and SpeI, gel extraction and gel purification<br> |
| + | · Ligation of BBa_B0015 cut on XbaI and PstI into pSB1C3-comX-myc backbone cut on SpeI and PstI<br> | ||
| + | · Transformation of pSB1C3-comX-myc.B0015 ligation product<br> | ||
| + | · Ligation of comX-myc cut on EcoRI-HF and SpeI into pSB1C3-B0015 backbone cut on EcoRI-HF and XbaI<br> | ||
| + | · Transformation of pSB1C3-B0015.comX-myc ligation product<br> | ||
| + | · Sequencing of comX-myc and comW-FLAG<br> | ||
| + | · Inoculation of comX-myc<br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 5</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Sequencing result failed<br> | ||
| + | · Transformation of pSB1C3-B0015.comX-myc ligation product failed<br> | ||
| + | · Digestion check of comX-myc on StyI<br> | ||
| + | · Colony PCR of comX-myc<br> | ||
| + | · Inoculation of comX-myc<br> | ||
| + | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 6</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Digestion check of comX-myc on XbaI and PstI<br> | ||
| + | · Gel check of comX-myc PCR product (no PCR product present)<br> | ||
| + | · Sequencing of comW-FLAG using VF2 and VR<br> | ||
| + | · Digestion of comX-myc on StyI<br> | ||
| + | · <br> | ||
| + | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 7</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Digestion of comX-myc on StyI<br> | ||
| + | · Digestion check of comX on SphI and NcoI<br> | ||
| + | · Digestion check of comW on XmnI and NcoI<br> | ||
· <br> | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | <br><b>Aug 8</b> <br> | ||
| + | Wet lab<br> | ||
| + | · Digestion of comW-FLAG on EcoRI-HF and SpeI, gel extraction and gel purification<br> | ||
| + | · Digestion of comW-FLAG on SpeI and PstI-HF, digestion clean-up<br> | ||
| + | · Sequencing of comX-myc succeeded but mutation at EcoRI site<br> | ||
| + | · Digestion check of comX-myc at EcoRI-HF and PstI-HF<br> | ||
| + | · Ligation of comX and comW into dephosporylated pSB1C3-K880005<br> | ||
| + | · Transformation of comX and comW ligation product<br> | ||
| + | · Digestion of comX-myc at EcoRI-HF and SpeI-HF<br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
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<a href="#" class="head">Week 2</a> | <a href="#" class="head">Week 2</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br><b>Aug | + | <br><b>Aug 11</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Digestion check of comX-myc and comW-FLAG on XbaI and PstI-HF<br> | ||
| + | · Gel purification of comX-myc digested on EcoRI-HF and SpeI<br> | ||
| + | · Ligation of comX-myc cut on EcoRI-HF and SpeI into pSB1C3-B0015 cut on EcoRI-HF and XbaI<br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 12</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Digestion check of pSB1C3-K880005.comX and pSB1C3-K880005.comW-FLAG on XbaI and PstI-HF<br> | ||
| + | · Dilution of comW-FLAG<br> | ||
| + | · Re-digestion of BBa_B0015 on EcoRI-HF and XbaI, digestion clean-up<br> | ||
| + | · Re-ligation of pSB1C3-B0015.comX-myc<br> | ||
| + | · Transformation of pSB1C3-B0015.comX-myc ligation product<br> | ||
| + | · Inoculation of pSB1C3-K880005.comX-myc.B0015 and pSB1C3-K880005.comX-myc<br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 13</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Digestion check of pSB1C3-B0015.comX-myc and pSB1C3-K880005.comX-myc.B0015 on XbaI and PstI-HF<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 14</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Digestion check of pSB1C3-K880005.comX-myc.B0015 on XbaI and PstI-HF<br> | ||
| + | · Transformation of ligated product pSB1C3-K880005.comX-myc.B0015<br> | ||
| + | · Dilution of comW-FLAG<br> | ||
| + | · Digestion of comW-FLAG on EcoRI-HF and SpeI-HF<br> | ||
| + | · Ligation of comW-FLAG into pSB1C3-B0015 backbone<br> | ||
| + | · Re-digestion of comW-FLAG on EcoRI-HF and SpeI-HF<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | <br><b>Aug 15</b> <br> | ||
| + | Wet lab<br> | ||
| + | · Digestion of pSB1C3-K880005 on SpeI-HF and PstI-HF, digestion clean-up<br> | ||
| + | · Colony PCR of comW-FLAG using Taq DNA Polymerase<br> | ||
| + | · Digestion of pSB1C3-B0015 on EcoRI-HF and XbaI<br> | ||
| + | · Digestion of pSB1C3-K880005.comX-myc on EcoRI-HF and SpeI-HF<br> | ||
| + | · Digestion of pSB1C3-B0015 on EcoRI-HF and XbaI, digestion clean-up<br> | ||
| + | · Digestion of pSB1C3-K880005.comX-myc on EcoRI-HF and SpeI-HF, gel extraction and gel purification<br> | ||
| + | · Overnight ligation of BBa_B0015 into pSB1C3-K880005.comX-myc backbone at 16°C<br> | ||
| + | · Transformation of pSB1C3-K880005.comX-myc.B0015 ligation product<br> | ||
| + | · Ligation of comX-myc into pSB1C3-B0015 backbone<br> | ||
| + | · Inoculation of pSB1C3-K880005.comW-FLAG and pSB1C3-K880005.comX-myc.B0015<br> | ||
| + | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
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<a href="#" class="head">Week 3</a> | <a href="#" class="head">Week 3</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br><b>Aug | + | <br><b>Aug 18</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Transformation of ligated pSB1C3-K880005.comX-myc<br> | ||
| + | · Ligation of comW-FLAG into pSB1C3-K880005 backbone, comX-myc into pSB1C3-K880005 backbone, BBa_B0015 into pSB1C3-K880005.comX-myc backbone<br> | ||
| + | · Digestion check of pSB1C3-K880005.comW-FLAG on EcoRI-HF and SpeI-HF<br> | ||
| + | · Digestion check of pSB1C3-K880005.comW-FLAG on SpeI-HF and PstI-HF<br> | ||
| + | · Digestion of pSB1C3-K880005.comX-myc on EcoRI-HF and SpeI-HF, gel extraction and gel purification<br> | ||
| + | · Inoculation of pSB1C3-K880005.comX-myc and pSB1C3-B0015<br> | ||
| + | · Digestion of pSB1C3-K880005.comX-myc on SpeI-HF and PstI-HF, digestion clean-up<br> | ||
| + | · Digestion check of pSB1C3-K880005.comX-myc.B0015 on XbaI and PstI-HF<br> | ||
| + | · Digestion of pSB1C3-K880005 on SpeI-HF and PstI-HF, digestion clean-up<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | <br><b>Aug 19</b> <br> | ||
| + | Wet lab<br> | ||
| + | · Digestion of pSB1C3-B0015 on EcoRI-HF and XbaI, digestion clean-up<br> | ||
| + | · Digestion check of comX-myc on XbaI and PstI-HF<br> | ||
| + | · Ligation of pSB1C3-K880005.comX-myc cut on EcoRI-HF and SpeI-HF iinto pSB1C3-B0015 backbone cut on EcoRI-HF and XbaI<br> | ||
| + | · Inoculation of pSB1C3-K880005.comW-FLAG and pSB1C3-K880005.comX-myc.B0015<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 20</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Digestion check of pSB1C3-K880005.comW-FLAG on EcoRI-HF and SpeI-HF, gel extraction and gel purification<br> | ||
| + | · Digestion check of pSB1C3-K880005.comX-myc.B0015 on XbaI and PstI-HF<br> | ||
| + | · Digestion check of pSB1C3-K880005.comX-myc.B0015 on SphI and SpeI-HF<br> | ||
| + | · Digestion check of pSB1C3-K880005.comX-myc on XbaI and PstI<br> | ||
| + | · Inoculation of pSB1C3-K880005.comX-myc.B0015 and pSB1C3-K880005.comW-FLAG<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 21</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Digestion check of pSB1C3-K880005.comW-FLAG on EcoRI-HF and SpeI-HF<br> | ||
| + | · Digestion check of pSB1C3-K880005.comX-myc.B0015 on XbaI and PstI-HF<br> | ||
| + | · Ligation of pSB1C3-K880005.comW-FLAG cut on EcoRI-HF and SpeI-HF into pSB1C3-B0015 backbone cut on EcoRI-HF and XbaI<br> | ||
| + | · Colony PCR of pSB1C3-B0015 and reverse comX-myc using Taq DNA Polymerase<br> | ||
| + | · Digestion of comW-FLAG on NotI-HF<br> | ||
| + | · Digestion of comW-FLAG on EcoRI-HF and SpeI-HF<br> | ||
| + | · Inoculation of pSB1C3-K880005.comW-FLAG<br> | ||
| + | · Transformation pSB1C3-K880005.comW-FLAG ligation product and pSB1C3-K880005.comX-myc.B0015<br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 22</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Digestion check of comW-FLAG on EcoRI-HF and SpeI-HF<br> | ||
| + | · Digestion of pSB1C3-K880005.comX-myc.B0015 on EcoRI-HF and SpeI-HF<br> | ||
| + | · Inoculation pSB1C3-K880005.comX-myc.B0015<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
· <br> | · <br> | ||
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<a href="#" class="head">Week 4</a> | <a href="#" class="head">Week 4</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br><b>Aug | + | <br><b>Aug 25</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Ligation of pSB1C3-K880005.comW-FLAG cut on EcoRI-HF and SpeI-HF into pSB1C3-B0015 backbone cut on EcoRI-HF and XbaI | ||
| + | Digestion check of pSB1C3-K880005.comX-myc.B0015 on XbaI and PstI-HF | ||
| + | Transformation of pSB1C3-K880005.comW-FLAG | ||
| + | Digestion of pSB1C3-K880005.comX-myc.B0015 on SpeI-HF and PstI-HF, gel extraction and gel purification | ||
| + | Inoculation of pSB1C3-K880005.comX-myc.B0015 for glycerol stock<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
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· <br> | · <br> | ||
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· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 26</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Digestion of pSB1C3-K880005.comX-myc.B0015 on SpeI-HF and PstI-HF, digestion clean-up<br> | ||
| + | · Ligation of Combox + GFP cut on XbaI and PstI-HF into pSB1C3-K880005.comX-myc.B0015 backbone cut on SpeI-HF and PstI-HF<br> | ||
| + | · Digestion of BBa_E0240 100 bp on XbaI and PstI-HF, gel extraction and gel purification<br> | ||
| + | · Inoculation of pSB1C3-K880005.comW-FLAG.B0015<br> | ||
| + | · Transformation of pSB1C3-K880005.comW-FLAG<br> | ||
| + | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 27</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Digestion check of pSB1C3-K880005.comW-FLAG on XbaI and PstI-HF, gel extraction and gel purification<br> | ||
| + | · Sequencing of pSB1C3-K880005.comW-FLAG.B0015<br> | ||
| + | · Ligation of pSB1C3-K880005.comW-FLAG into pSB1C3-B0015 backbone and Combox + GFP into pSB1C3-K880005.comX-myc.B0015 backbone <br> | ||
| + | · Transformation of pSB1C3-K880005.comW-FLAG<br> | ||
| + | · Inoculation of pSB1C3-K880005.comW-FLAG.B0015 and pSB1C3-K880005.comW-FLAG<br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 28</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Digestion check of pSB1C3-K880005.comW-FLAG.B0015 on XbaI and PstI-HF, gel extraction and gel purification<br> | ||
| + | · Digestion check of pSB1C3-K880005.comW-FLAG on EcoRI-HF and SpeI-HF, gel extraction and gel purification<br> | ||
| + | · Ligation of Combox + GFP into pSB1C3-K880005.comX-myc.B0015 backbone<br> | ||
| + | · Inoculation of pSB1C3-K880005.comW-FLAG.B0015 and pSB1C3-K880005.comW-FLAG<br> | ||
| + | · Ligation of pSB1C3-K880005.comW-FLAG into pSB1C3-B0015 backbone<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | <br><b>Aug 29</b> <br> | ||
| + | Wet lab<br> | ||
| + | · Digestion check of pSB1C3-K880005.comW-FLAG.B0015 on XbaI and PstI-HF<br> | ||
| + | · Digestion check of pSB1C3-K880005.comW-FLAG on EcoRI-HF and SpeI-HF<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
Revision as of 16:04, 6 October 2014
Notebook
|
Throughout the course of our project, we have continuously recorded our daily work, such as wetlab activities and research findings. The notebook for Pneuumonsensor and Riboregulator are displayed separately. PneumosensorRiboregulator |
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