Team:Bielefeld-CeBiTec/Notebook/Organisms

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       <a href="https://static.igem.org/mediawiki/2014/2/25/Bielefeld-CeBiTec_2014-09-29_Ecoli.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/2/25/Bielefeld-CeBiTec_2014-09-29_Ecoli.png" width="250px"></a>
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       <br><font size="1" style="text-align:center;">Scanning electron micrograph of Escherichia coli, grown in culture and adhered to a cover slip.</font>
       <br><font size="1" style="text-align:center;">Scanning electron micrograph of Escherichia coli, grown in culture and adhered to a cover slip.</font>
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       <a href="https://static.igem.org/mediawiki/2014/0/02/Bielefeld-CeBiTec_2014-09-29_Llactis.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/0/02/Bielefeld-CeBiTec_2014-09-29_Llactis.png" width="250px"></a>
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<br><font size="1" style="text-align:center;">Scanning Electron Micrograph of Enterococcus faecalis</font>
<br><font size="1" style="text-align:center;">Scanning Electron Micrograph of Enterococcus faecalis</font>
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       <a href="https://static.igem.org/mediawiki/2014/a/a5/Bielefeld-CeBiTec_2014-09-29_Hneapolitanus.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/a/a5/Bielefeld-CeBiTec_2014-09-29_Hneapolitanus.png" width="250px"></a>
       <a href="https://static.igem.org/mediawiki/2014/a/a5/Bielefeld-CeBiTec_2014-09-29_Hneapolitanus.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/a/a5/Bielefeld-CeBiTec_2014-09-29_Hneapolitanus.png" width="250px"></a>
       <br><font size="1" style="text-align:center;">(A) Electron micrograph of Halothiobacillus neapolitanus cells, arrows highlight carboxysomes. (B) Image of intact carboxysomes isolated from H. neapolitanus. Scale bars indicate 100 nm.</font>
       <br><font size="1" style="text-align:center;">(A) Electron micrograph of Halothiobacillus neapolitanus cells, arrows highlight carboxysomes. (B) Image of intact carboxysomes isolated from H. neapolitanus. Scale bars indicate 100 nm.</font>
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       <a href="https://static.igem.org/mediawiki/2014/8/88/Bielefeld-CeBiTec_2014-09-29_Bsubtilis.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/8/88/Bielefeld-CeBiTec_2014-09-29_Bsubtilis.png" width="250px"></a>
       <a href="https://static.igem.org/mediawiki/2014/8/88/Bielefeld-CeBiTec_2014-09-29_Bsubtilis.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/8/88/Bielefeld-CeBiTec_2014-09-29_Bsubtilis.png" width="250px"></a>
       <br><font size="1" style="text-align:center;">The Bacterium Bacillus subtilis taken with a Tecnai T-12 TEM. Taken by Allon Weiner, The Weizmann Institute of Science, Rehovot, Israel. 2006.</font>
       <br><font size="1" style="text-align:center;">The Bacterium Bacillus subtilis taken with a Tecnai T-12 TEM. Taken by Allon Weiner, The Weizmann Institute of Science, Rehovot, Israel. 2006.</font>
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       <a href="https://static.igem.org/mediawiki/2014/2/20/Bielefeld-CeBiTec_2014-09-29_Selongatus.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/2/20/Bielefeld-CeBiTec_2014-09-29_Selongatus.png" width="250px"></a>
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       <br><font size="1" style="text-align:center;">Synechococcus PCC 7002 in DIC microscopy. Imaging was performed with the Olympus BX61 microscope and a UPlanSApo 100× NA 1.40 oil immersion objective (Olympus). Pictures were acquired at room temperature in water with a camera (SPOT; Diagnostic Instruments, Inc.) using MetaMorph software (MDS Analytical Technologies).</font>
       <br><font size="1" style="text-align:center;">Synechococcus PCC 7002 in DIC microscopy. Imaging was performed with the Olympus BX61 microscope and a UPlanSApo 100× NA 1.40 oil immersion objective (Olympus). Pictures were acquired at room temperature in water with a camera (SPOT; Diagnostic Instruments, Inc.) using MetaMorph software (MDS Analytical Technologies).</font>
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       <a href="https://static.igem.org/mediawiki/2014/4/4a/Bielefeld-CeBiTec_2014-09-29_Asuccinogenes.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/4/4a/Bielefeld-CeBiTec_2014-09-29_Asuccinogenes.png" width="250px"></a>
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       <br><font size="1" style="text-align:center;"><i>Actinobacillus lignieresi</i>. Gram stain.</font>
       <br><font size="1" style="text-align:center;"><i>Actinobacillus lignieresi</i>. Gram stain.</font>
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       <a href="https://static.igem.org/mediawiki/2014/9/92/Bielefeld-CeBiTec_2014-09-29_Cglutamicum.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/9/92/Bielefeld-CeBiTec_2014-09-29_Cglutamicum.png" width="250px"></a>
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       <br><font size="1" style="text-align:center;">Phase contrast of <i>Corynebacterium glutamicum</i></font>
       <br><font size="1" style="text-align:center;">Phase contrast of <i>Corynebacterium glutamicum</i></font>
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       <a href="https://static.igem.org/mediawiki/2014/6/60/Bielefeld-CeBiTec_2014-09-30_Bmethanolicus.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/6/60/Bielefeld-CeBiTec_2014-09-30_Bmethanolicus.png" width="250px"></a>
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       <br><font size="1" style="text-align:center;">microscopic image of Bacillus subtilis (ATCC 6633). Gram staining, magnification:1,000. The oval unstained structures are spores.</font>
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       <br><font size="1" style="text-align:center;">Microscopic image of Bacillus subtilis (ATCC 6633). Gram staining, magnification:1,000. The oval unstained structures are spores.</font>
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Revision as of 19:02, 4 October 2014


Organisms

Escherichia coli
Escherichia coli

Scanning electron micrograph of Escherichia coli, grown in culture and adhered to a cover slip.


The strain KRX is used for all approaches. Coding sequences of this organism were used for different purposes:

  • frd - Fumarate reductase
  • Cytochromes
DomainBacteria
PhylumProteobacteria
ClassGammaproteobacteria
OrderEnterobacteriales
FamilyEnterobacteriaceae
GenusEscherichia
SpeciesE. coli
Lactococcus lactis
Lactococcus lactis

Scanning Electron Micrograph of Enterococcus faecalis


Coding sequences of this organism were used for the isobutanol production:

  • kivD - α-ketoisovalerate decarboxylase
  • adhA - Alcoholdehydrogenase
DomainBacteria
PhylumFirmicutes
ClassBacilli
OrderLactobacillales
FamilyStreptococcaceae
GenusLactococcus
SpeciesL. lactis
Halothiobacillus neapolitanus
Halothiobacillus neapolitanus

(A) Electron micrograph of Halothiobacillus neapolitanus cells, arrows highlight carboxysomes. (B) Image of intact carboxysomes isolated from H. neapolitanus. Scale bars indicate 100 nm.


Coding sequences of this organism were used for the carboxysome:

  • sap - shell associated protein
  • can - carbonic anhydrase
  • csoS1-4 - shell protein
  • csoS1D - pore protein
DomainBacteria
PhylumProteobacteria
Classγ-Proteobacteria
OrderChromatiales
FamilyHalothiobacillaceae
GenusHalothiobacillus
SpeciesH. neapolitanus
Bacillus subtilis
Bacillus subtilis

The Bacterium Bacillus subtilis taken with a Tecnai T-12 TEM. Taken by Allon Weiner, The Weizmann Institute of Science, Rehovot, Israel. 2006.


A coding sequence of this organism was used for the isobutanol production:

  • alsS - α-acetolactate synthase
DomainBacteria
PhylumFirmicutes
ClassBacilli
OrderBacillales
FamilyBacillaceae
GenusBacillus
SpeciesB. subtilis
Synechococcus elongatus
Synechococcus elongatus

Synechococcus PCC 7002 in DIC microscopy. Imaging was performed with the Olympus BX61 microscope and a UPlanSApo 100× NA 1.40 oil immersion objective (Olympus). Pictures were acquired at room temperature in water with a camera (SPOT; Diagnostic Instruments, Inc.) using MetaMorph software (MDS Analytical Technologies).


A coding sequence of this organism was used for the carbon dioxide fixation:

  • RuBisCO - Ribulose 1,5-bisphosphate carboxylase / oxygenase
  • prkA - Phosphoribulokinase
DomainBacteria
PhylumCyanobacteria
ClassOscillatoriophycideae
OrderChroococcales
GenusSynechococcus
SpeciesS. elongatus
Actinobacillus succinogenes
Actinobacillus succinogenes

Actinobacillus lignieresi. Gram stain.


A coding sequence of this organism was used for the transport of reduction equivalents:

  • Asuc_1813/1814/1815/1816 - fumarate reductase
DomainBacteria
PhylumProteobacteria
Classγ-Proteobacteria
OrderPasteurellales
FamilyPasteurellaceae
GenusActinobacillus
SpeciesA. succinogenes
Geobacter sulfurreducens
Geobacter sulfurreducens


A coding sequence of this organism was used for the transport of electrons:

  • Cytochromes
DomainBacteria
PhylumProteobacteria
Subphylumδ / ε-Proteobacteria
Classδ-Proteobacteria
OrderDesulfuromonadales
FamilyGeobacteraceae
GenusGeobacter
SpeciesG. sulfurreducens
Corynebacterium glutamicum
Corynebacterium glutamicum

Phase contrast of Corynebacterium glutamicum


A coding sequence of this organism was used for an enzyme assay:

  • pgk - Phosphoglycerate kinase
DomainBacteria
PhylumActinobacteria
ClassActinobacteridae
OrderActinomycetales
SuborderCorynebacterineae
FamilyCorynebacteriaceae
GenusCorynebacterium
SpeciesC. glutamicum
Bacillus methanolicus
Bacillus methanolicus

Microscopic image of Bacillus subtilis (ATCC 6633). Gram staining, magnification:1,000. The oval unstained structures are spores.


A coding sequence of this organism was used for carbon dioxide fixation:

  • glpX - Sedoheptulose 1,7-bisphosphatase
DomainBacteria
PhylumFirmicutes
ClassBacilli
OrderBacillales
FamilyBacillaceae
GenusBacillus
SpeciesB. methanolicus

References

  • http://en.wikipedia.org/wiki/Escherichia_coli
  • http://nl.wikipedia.org/wiki/Melkzuurbacteri
  • http://en.wikipedia.org/wiki/Carboxysome
  • http://en.wikipedia.org/wiki/Bacillus_subtilis
  • http://en.wikipedia.org/wiki/Synechococcus
  • http://de.wikipedia.org/wiki/Actinobacillus
  • http://de.wikipedia.org/wiki/Corynebacterium_glutamicum
  • http://en.wikipedia.org/wiki/Bacillus

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