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Team:Hong Kong HKUST/wetlab/notebook
From 2014.igem.org
(Difference between revisions)
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<a href="#" class="head">Week 4</a> | <a href="#" class="head">Week 4</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br> <b> July | + | <br> <b> July 21 </b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Gel purification of comX and comX-myc<br> | ||
| + | · Ligation of comX-myc into dephosporylated pSB1C3-K880005 backbone with 1:4, 1:7, 1:10 ratio<br> | ||
| + | · Ligation of comW-FLAG into dephosporylated pSB1C3-K880005 backbone with 1:4, 1:7, 1:10 ratio<br> | ||
| + | · Ligation of comX into dephosporylated pSB1C3-K880005 backbone with 1:3 and 1:5 ratio<br> | ||
| + | · Ligation of comW into dephosporylated pSB1C3-K880005 backbone with 1:4, 1:7, 1:10 ratio<br> | ||
| + | · Colony PCR of pSB1C3-K880005.comW-FLAG using Taq DNA Polymerase<br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 493: | Line 499: | ||
· <br> | · <br> | ||
| - | <br> <b> July | + | <br> <b> July 22</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Colony PCR of comX, comW, comX-myc, comW-FLAG and use BBa_J04450 as the positive control using Taq DNA Polymerase (no PCR product present)<br> | ||
| + | · Inoculation of comX, comW, comX-myc, comW-FLAG<br> | ||
| + | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
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· <br> | · <br> | ||
| - | <br> <b> July | + | <br> <b> July 23</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Digestion check of comX, comW, comX-myc, comW-FLAG colony PCR products on XbaI and SpeI<br> | ||
| + | · Inoculation of comW-FLAG<br> | ||
| + | · <br> | ||
| + | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br> <b> July | + | |
| + | <br> <b> July 24</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| + | · Digestion check of pSB1C3-K880005.comW-FLAG on XbaI and SpeI-HF<br> | ||
| + | · Ligation of comW-FLAG into dephosporylated pSB1C3-K880005 backbone<br> | ||
| + | · Ligation of comX-myc into dephosporylated pSB1C3-K880005 backbone<br> | ||
| + | · PCR of comX-myc and comX using Vent DNA Polymerase, gel extraction and gel purification<br> | ||
| + | · Digestion of comX-myc and comX on XbaI and PstI-HF<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | <br> <b> July 25</b> <br> | ||
| + | Wet lab<br> | ||
| + | · Colony PCR of comX-myc using Taq DNA Polymerase<br> | ||
| + | · Gel check of comX-myc PCR product (no PCR product present)<br> | ||
| + | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 518: | Line 546: | ||
<a href="#" class="head">Week 5</a> | <a href="#" class="head">Week 5</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br> <b> July | + | <br> <b> July 28</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Colony PCR of comX-myc using Taq DNA Polymerase<br> | ||
| + | · Colony PCR of comw-FLAG using Taq DNA Polymerase<br> | ||
| + | · Inoculation of comX-myc and comW-FLAG<br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 525: | Line 556: | ||
· <br> | · <br> | ||
| - | <br> <b> July | + | <br> <b> July 29</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Digestion check of comX-myc and comW-FLAG on XbaI and PstI-HF<br> | ||
| + | · Transformation of comX-myc and comW-FLAG<br> | ||
| + | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 532: | Line 566: | ||
· <br> | · <br> | ||
| - | <br> <b> July | + | <br> <b> July 30</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Digestion check of comX-myc and comW-FLAG<br> | ||
| + | · Inoculation of comX-myc and comW-FLAG <br> | ||
| + | · Streaking plates of comX-myc and comW-FLAG<br> | ||
| + | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br> <b> July | + | <br> <b> July 31</b> <br> |
Wet lab<br> | Wet lab<br> | ||
| + | · Digestion check of comX-myc and comW-FLAG on XbaI and PstI-HF<br> | ||
| + | · Redigest comW-FLAG on XmnI and NcoI<br> | ||
| + | · PCR sequencing for comW and comX<br> | ||
| + | · Ethanol precipitation for comW and comX<br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
Revision as of 14:10, 3 October 2014
Notebook
|
Throughout the course of our project, we have continuously recorded our daily work, such as wetlab activities and research findings. The notebook for Pneuumonsensor and Riboregulator are displayed separately. PneumosensorRiboregulator |
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