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Team:KIT-Kyoto/Test/Notebook/Labnote
From 2014.igem.org
(Difference between revisions)
| Line 93: | Line 93: | ||
<table class="materials"> | <table class="materials"> | ||
<tr> | <tr> | ||
| - | < | + | <th rowspan="2">Host</th> |
| - | < | + | <th colspan="2">Sample</th> |
| - | < | + | <th rowspan="2">Enzyme</th> |
| - | < | + | <th rowspan="2">Buffer</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | < | + | <th>Vector</th> |
| - | < | + | <th>Insert</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Revision as of 06:20, 2 October 2014
LabNote
June
June 10, 2014
OKAMOTO
Miniprep
| Host | Sample | |
|---|---|---|
| Vector | Insert | |
| BL21(DE3) | pGEX6P-2 | CuMTSE1 |
| BL21(DE3) | pGEX6P-2 | CuMTSE2 |
| BL21(DE3) | pGEX6P-2 | CuMTS3 |
| BL21(DE3) | pGEX6P-2 | CuMTS62 |
Restriction Digest
| Host | Sample | Enzyme | Buffer | |
|---|---|---|---|---|
| Vector | Insert | |||
| BL21(DE3)PlysS | pGEX6P-2 | CuMTSE1 | EcoR1 | Buffer H |
| BL21(DE3)PlysS | pGEX6P-2 | CuMTSE2 | EcoR1 | Buffer H |
| BL21(DE3)PlysS | pGEX6P-2 | CuMTS3 | EcoR1 | Buffer H |
| BL21(DE3)PlysS | pGEX6P-2 | CuMTSE62 | EcoR1 | Buffer H |
| BL21(DE3)PlysS | pGEX6P-2 | CuMTSE1 | Sal1, Not1 | Buffer H |
| BL21(DE3)PlysS | pGEX6P-2 | CuMTSE2 | Sal1, Not1 | Buffer H |
| BL21(DE3)PlysS | pGEX6P-2 | CuMTS3 | Sal1, Not1 | Buffer H |
| BL21(DE3)PlysS | pGEX6P-2 | CuMTSE62 | Sal1, Not1 | Buffer H |
July
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August
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October
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