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Team:Caltech/TXTL
From 2014.igem.org
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| + | TXTL is a cell-free transcription translation system that allows for rapid prototyping of biological circuits. | ||
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| - | + | We characterized the strengths of a family of constitutive promoters in TXTL. We sought to test the Anderson promoters, which were contributed in 2006 by Berkeley's iGEM team. The first figure shows the reported RFP fluorescence values measured <i>in vivo</i> by Anderson <i>et al</i> for different constitutive promoter constructs. The second figure shows the <i>in vitro</i> RFP fluorescence values measured for the same promoters using TXTL. <br> | |
<img src="https://static.igem.org/mediawiki/2014/1/1b/BerkiGEM2006_promoter.png" width="1000px"> | <img src="https://static.igem.org/mediawiki/2014/1/1b/BerkiGEM2006_promoter.png" width="1000px"> | ||
Revision as of 17:14, 1 October 2014
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| Introduction to TXTL | |||||||||||||
| Anderson Promoters | |||||||||||||
We characterized the strengths of a family of constitutive promoters in TXTL. We sought to test the Anderson promoters, which were contributed in 2006 by Berkeley's iGEM team. The first figure shows the reported RFP fluorescence values measured in vivo by Anderson et al for different constitutive promoter constructs. The second figure shows the in vitro RFP fluorescence values measured for the same promoters using TXTL. 
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