Contributions

From the project choice to the wiki design and presentation, all the work has been done by students, be it the design of the constructs, the experimental work or the modeling.

Even though work on the bench and behind the bench was done by each student, every student had the responsibility of a particular task. Daniel was responsible for the lab, Maximilian for the Sponsoring and the chip design, Nadine for the administrative work, Stefanie for Human Practice and the lab practical work; Claude for the Wiki and for the diffusion model, Elise for the model, Sumana for the iGEM site watch and for model simulations.

We would like to thank our advisors and instructors for all the helpful advice they gave us throughout the project.

Acknowledgements

3D-Printing & PDMS Chip

Mario Mauerer shared his knowledge and time to teach us in 3D-printing. Thanks to him we were able to produce our millifluidic devices.

Gregor Schmidt and Arne Wehling helped with PDMS preparation and shared their knowledge about microfluidic chip design.

Flow cytometry

We highly appreciated the introduction and support Verena Jäggin provided us in flow cytometry. Her help allowed us to analyse the expression of the recombinase TP910 in more details.

pPaB strains

Andreas Bosshart provided all the pPaB strains we used for plasmid construction and never hesitated to help us in case of troubles. We are very grateful for that.

Gibson assembly

Dr. Tania Roberts taught us how to conduct Gibson assembly. She did not only provide us with materials and protocols, but also helped us to elaborate important texts in English. We could profit a lot, thanks.

Alginate beads

Steven Schmitt showed us how to produce alginate beads. He advised us in our project and let us use his materials and protocols. We are very grateful for his patience and generosity.

Safety

Thanks to Niels Bürckert for helpful discussions concerning the safety form.