Revision as of 14:07, 28 September 2014

Journal

Protocols

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Strains & Constructs

Kits & Enzymes

Primer

Acronyms

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Additional

July

rMFC

CO₂ fixation

Isobutanol

Biosafety

General

Week 1 07/07 - 07/13

This week we amplified the pSB1C3 backbone of FumA and FumBCD to use it for Gibson Assembly afterwards

Week 2 07/14 - 07/20

Week 3 07/21 - 07/27

Week 4 07/28 - 08/03

@@ Line 56: / Line 56: @@
 <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PCR" target="_blank">PCR amplification </a>(<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_Fum_A" target="_blank"> pSB1C3_pre_Fum_A</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_Fum_A " target="_blank"> pSB1C3_suf_Fum_A </a>)</li>
 <ul>
-<li>Annealing temperature: -- &deg;C</li>
+<li>Annealing temperature: 55 &deg;C</li>
 <li>Bands are as expected (2,07 kb)</li>
+</ul>
+<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PCR" target="_blank">PCR amplification </a>(<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_Fum_BCD" target="_blank"> pSB1C3_pre_Fum_BCD</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_Fum_BCD " target="_blank"> pSB1C3_suf_Fum_BCD </a>)</li>
+<ul>
+<li>Annealing temperature: 55 &deg;C</li>
+<li>Bands are as expected (2,07 kb)</li>
+</ul>
+<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#DNAPurificationbyCentrifugation" target="_blank">PCR purification</a> of backbones</li>
+</ul>
+</ul>
+</ul>
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        </div>