Team:Evry/Interlab Study/09-17-2014

From 2014.igem.org

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<br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2>
<br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2>
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pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation 16h-18h at 37°C.
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<h2>Other constructions of the Anderson library of constitutive promoters</h2>
<h2>Other constructions of the Anderson library of constitutive promoters</h2>
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<i>PSB1C3+E0240+J23107 and PSB1C3+E0240+J23105</i>: <br> pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation overnight at 37°C.
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<i>PSB1C3+E0240+J23100</i>: <br>
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Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h).
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<img src="" alt="PLAN DE PLAQUE" />
<br>
<br>
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<i>PSB1C3+E0240+J23103</i>: <br>
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Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h).
 +
<img src="" alt="PLAN DE PLAQUE" />
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<br>
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<i>PSB1C3+E0240+J23115</i>: <br>
 +
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h).
 +
<img src="" alt="PLAN DE PLAQUE" />
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<br>
 +
 +
<i>PSB1C3+E0240+J23110</i>: <br>
 +
pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation 16h-18h at 37°C.<br>
 +
 +
<i>PSB1C3+E0240+J23107 and PSB1C3+E0240+J23105</i>: <br>
 +
pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation overnight at 37°C.<br>
 +
<br>
 +
<span class="cd-date">Sep 17</span>  
<span class="cd-date">Sep 17</span>  

Revision as of 19:34, 19 September 2014

Picture

Construction n°1: PSB1C3 with I20260




Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010


pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation 16h-18h at 37°C.

Other constructions of the Anderson library of constitutive promoters


PSB1C3+E0240+J23100:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h). PLAN DE PLAQUE
PSB1C3+E0240+J23103:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h). PLAN DE PLAQUE
PSB1C3+E0240+J23115:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h). PLAN DE PLAQUE
PSB1C3+E0240+J23110:
pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23107 and PSB1C3+E0240+J23105:
pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation overnight at 37°C.

Sep 17