Team:Evry/Interlab Study/09-17-2014
From 2014.igem.org
(Difference between revisions)
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<br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2> | <br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2> | ||
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+ | pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation 16h-18h at 37°C. | ||
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<h2>Other constructions of the Anderson library of constitutive promoters</h2> | <h2>Other constructions of the Anderson library of constitutive promoters</h2> | ||
<br/> | <br/> | ||
- | <i>PSB1C3+E0240+ | + | |
+ | <i>PSB1C3+E0240+J23100</i>: <br> | ||
+ | Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h). | ||
+ | <img src="" alt="PLAN DE PLAQUE" /> | ||
<br> | <br> | ||
+ | |||
+ | <i>PSB1C3+E0240+J23103</i>: <br> | ||
+ | Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h). | ||
+ | <img src="" alt="PLAN DE PLAQUE" /> | ||
+ | <br> | ||
+ | |||
+ | <i>PSB1C3+E0240+J23115</i>: <br> | ||
+ | Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h). | ||
+ | <img src="" alt="PLAN DE PLAQUE" /> | ||
+ | <br> | ||
+ | |||
+ | <i>PSB1C3+E0240+J23110</i>: <br> | ||
+ | pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation 16h-18h at 37°C.<br> | ||
+ | |||
+ | <i>PSB1C3+E0240+J23107 and PSB1C3+E0240+J23105</i>: <br> | ||
+ | pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation overnight at 37°C.<br> | ||
+ | <br> | ||
+ | |||
<span class="cd-date">Sep 17</span> | <span class="cd-date">Sep 17</span> |
Revision as of 19:34, 19 September 2014
Construction n°1: PSB1C3 with I20260
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation 16h-18h at 37°C.
Other constructions of the Anderson library of constitutive promoters
PSB1C3+E0240+J23100:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h).
PSB1C3+E0240+J23103:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h).
PSB1C3+E0240+J23115:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h).
PSB1C3+E0240+J23110:
pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23107 and PSB1C3+E0240+J23105:
pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation overnight at 37°C.
Sep 17