Team:Evry/Interlab Study/09-17-2014

Construction n°1: PSB1C3 with I20260

Construction n°2: PSB1C3 with J23101-E1010

Construction n°3: PSB1C3 with K823012-E1010

Pre-culture from glycerol stock in LB+Cam (1:1000). Incubation 16h-18h at 37°C.

Other constructions of the Anderson library of constitutive promoters

PSB1C3+E0240+J23100:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h).
OVERFLOW

PSB1C3+E0240+J23101:
Measure of the cells' growth and fluorecence on TECAN infiniteM200.
TECAN FAILED

PSB1C3+E0240+J23103:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h30-23h30).


PSB1C3+E0240+J23105:
pre-culture of the colony 5 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation overnight at 37°C.

PSB1C3+E0240+J23107:
pre-culture of the colony 5 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation overnight at 37°C.

PSB1C3+E0240+J23110:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23115:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (12h-19h).


PSB1C3+E0240+J23104, PSB1C3+E0240+J23112, PSB1C3+E0240+J23113: PCR colony of 6 colonies for each construction following protocol table 1 and 2.
Migration of the PCR products
PCR purification of clones with the good construction
A pre-culture was made for each colony to make a glycerol stock.

Sep 17