Team:Evry/Interlab Study/09-01-2014

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<h2>Constructions with other promoters</h2>
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<h2>Other constructions of the Anderson library of constitutive promoters</h2>
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PCR colony were performed on colonies from transformations of the 29th August. Used protocol was the same as Table 1 and 2 with the Q5 high fidelity enzyme.
PCR colony were performed on colonies from transformations of the 29th August. Used protocol was the same as Table 1 and 2 with the Q5 high fidelity enzyme.

Revision as of 16:50, 19 September 2014

Picture

Construction n°1: PSB1C3 with I20260




Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010



Other constructions of the Anderson library of constitutive promoters


PCR colony were performed on colonies from transformations of the 29th August. Used protocol was the same as Table 1 and 2 with the Q5 high fidelity enzyme.
PCR product of first colony of each transformation was loaded on 1% agarose gel and gel run 45 minutes at 110 mV.
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PCR product of BBa_J61002 plasmids containing promoters results.
3 ml LB culture were started from tested colonies which presented the right PCR product profile. Cultures were incubated overnight at 37°C. Sep 01

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