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Team:Evry/Interlab Study/08-23-2014
From 2014.igem.org
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<h2>Contruction n°1: PSB1C3 with I20260</h2> | <h2>Contruction n°1: PSB1C3 with I20260</h2> | ||
<br/>Digestion of PSB1C3 and PSB1K3-I20260 by EcoRI and PstI | <br/>Digestion of PSB1C3 and PSB1K3-I20260 by EcoRI and PstI | ||
| - | < | + | <ul> |
| - | <br/> | + | <li> 1 µl EcoRI-HF |
| + | <li> 1 µl PstI | ||
| + | <li> 2 µl buffer | ||
| + | <li> 0.2 µl BSA | ||
| + | <li> 13.7 or 76 µl respectively for I20260 and PSB1C3 | ||
| + | <li> 10.8 µl miliQ watter | ||
| + | </ul> | ||
| + | <br/>Incubation 45 minutes at 37°C. Denaturation step 20 minutes at 80 °C. | ||
| + | |||
<br/>Loading on a 1% agarose gel to perform an extraction from gel | <br/>Loading on a 1% agarose gel to perform an extraction from gel | ||
Revision as of 20:45, 17 September 2014
Contruction n°1: PSB1C3 with I20260
Digestion of PSB1C3 and PSB1K3-I20260 by EcoRI and PstI
- 1 µl EcoRI-HF
- 1 µl PstI
- 2 µl buffer
- 0.2 µl BSA
- 13.7 or 76 µl respectively for I20260 and PSB1C3
- 10.8 µl miliQ watter
Incubation 45 minutes at 37°C. Denaturation step 20 minutes at 80 °C.
Loading on a 1% agarose gel to perform an extraction from gel
Contruction n°2: PSB1C3 with J23101-E1010
Contruction n°3: PSB1C3 with K823012-E1010
Aug 23
