Team:The Tech Museum/Project

From 2014.igem.org

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<p>Our goal is to create an activity where Tech Museum visitors with no biology background can become part of our team and can engage in the hands-on experience of engineering a bacteria. In our project, they are guided through the transformation of a plasmid into e.coli and how we randomized the amount each of the three color reporters are expressed. When the transformed bacteria grow, each colony appears as a different color, essentially creating bacterial "pixels" representing a particular combination of the protein levels of each reporter. Visitors take these to a scanning station that uses computer-vision software to quantify the color and intensity of each colony.
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<p>Our goal is to enable Tech Museum visitors with no biology background to become part of our team by engineering bacteria hands-on.
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In our project, participants are guided through the transformation of e.coli with plasmids. We randomize the expression of the three color reporters, so when the transformed bacteria grow, colonies appear as a different colors, creating bacterial "pixels" representing a particular combination of reporter protein concentrations. Visitors then take the petri dishes to a scanning station that uses computer vision to quantify the color and intensity of each colony.
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This is the first time a museum has entered this competition and our team is prototyping ways we as an institution can offer novel activities for our community to both learn and develop the skills of future innovators. </p>
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This is the first time a museum has entered this competition. Our team is prototyping ways in which we as an institution can offer novel activities for our community to both learn and develop the skills of future innovators. </p>
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<h3>References </h3>
<h3>References </h3>

Revision as of 21:46, 8 September 2014



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Project Description

Content

Our goal is to enable Tech Museum visitors with no biology background to become part of our team by engineering bacteria hands-on. In our project, participants are guided through the transformation of e.coli with plasmids. We randomize the expression of the three color reporters, so when the transformed bacteria grow, colonies appear as a different colors, creating bacterial "pixels" representing a particular combination of reporter protein concentrations. Visitors then take the petri dishes to a scanning station that uses computer vision to quantify the color and intensity of each colony. This is the first time a museum has entered this competition. Our team is prototyping ways in which we as an institution can offer novel activities for our community to both learn and develop the skills of future innovators.


References

iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.

You can use these subtopics to further explain your project

  1. Overall project summary
  2. Project Details
  3. Materials and Methods
  4. The Experiments
  5. Results
  6. Data analysis
  7. Conclusions

It's important for teams to describe all the creativity that goes into an iGEM project, along with all the great ideas your team will come up with over the course of your work.

It's also important to clearly describe your achievements so that judges will know what you tried to do and where you succeeded. Please write your project page such that what you achieved is easy to distinguish from what you attempted.