Team:UCL/Science/Experiment

From 2014.igem.org

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     <div class="imagewc"><h1>Experiments</h1></div>
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     <div><h3>Experiments</h3></div>
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            <div class="jointb"><h3 style="margin:0px;">Web and Communications Team</h3></div>
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        <div class="floater"><h4 class="minimyzr" style="margin:0px;">Laboratory Team</h4></div>
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<div class="textTitle"><h3 class="widthCorrect">List of Experiments</h3></div>
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<li>
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<a href="/Team:UCL/experiments#Expt01">Experiment 01: Extraction of useful BioBrick plasmids from iGEM 2014 Distribution Kit</a>
 +
</li>
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  <!-- CONTENT GOES HERE -->
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<li>
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    <div class="hero-unit">
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<a href="/Team:UCL/experiments#Expt02">Experiment 02: Transforming <i>E. coli</i> with Azo-reductase plasmids</a>
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            <h2>About our project</h2>
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<a href="/Team:UCL/experiments#Expt03">Experiment 03: Diagnostic digest of azo-reductase plasmids</a>
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<li>
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<a href="/Team:UCL/experiments#Expt04">Experiment 04: Creation of azo-reductase BioBrick parts from plasmids</a>
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</li>
 +
                   
 +
<li>
 +
<a href="/Team:UCL/experiments#Expt05">Experiment 05: Diagnostic digest of azo-reductase BioBrick parts</a>
 +
</li>
 +
 
 +
<li>
 +
<a href="/Team:UCL/experiments#Expt06">Experiment  06: Assembling azo-reductase BioBrick Device(s)</a>
 +
</li>
 +
 
 +
<li>
 +
<a href="/Team:UCL/experiments#Expt07">Experiment 07: Characterisation of azo-reductase BioBrick devices</a>
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                <h1>Experiments</h1>
 
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                <p><h2>Quick Links:</h2>
 
-
                <strong>[CHECK these steps with: TO / AD / LB / DT]</strong>
 
-
                <ol>
 
-
                    <li><a href="/Team:UCL/experiments#Genes">Genes, Plasmids, and BioBricks</a> (Genes and Parts that we're working with)</li>
 
-
                    <li><a href="/Team:UCL/experiments#Expt00">Expt 00: Identifying and Confirming Necessary BioBricks from iGEM Parts Registry / Distribution</a> (ID, transform, miniprep (MP), digest, gel)</li>
 
-
                    <li><a href="/Team:UCL/experiments#Expt01">Expt 01: Isolating and Purifying Genes from Lisbon Plasmids</a> (Transform, MP)</li>
 
-
                    <li><a href="/Team:UCL/experiments#Expt02">Expt 02: Confirming Identity of Genes from Lisbon Plasmids</a> (Digest, gel)</li>
 
-
                    <li><a href="/Team:UCL/experiments#Expt03">Expt 03: Converting Lisbon Genes into BioBrick Part (Format)</a> (Primer design/order, (site-directed mutatgenesis) PCR)</li>
 
-
                    <li><a href="/Team:UCL/experiments#Expt04">Expt 04: Confirming Successful Conversion to BioBirck Format</a> (Subclone: digest, ligate, transform, MP, digest, gel)</li>
 
-
                    <li><a href="/Team:UCL/experiments#Expt05">Expt 05: Assembling BioBrick Device(s)</a> (Digest, ligate, transform, MP, digest, gel. Repeat ad infinitum...)</li>
 
-
                    <li><a href="/Team:UCL/experiments#Expt06">Expt 06: Characterisation- Azoreductase Assays</a> (See <a href="https://docs.google.com/document/d/1sU1CWSoi1Anydh2AkGb7ZnfdKoWgiAIJ7hat0gvctS0/edit?usp=sharing">Standard Operating Procedure</a> (SOP))</li>
 
                 </ol></p>
                 </ol></p>
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                <h2><a name="Genes">Genes, Plasmids, and BioBricks</a></h2>
 
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                    <br>[Internal hyperlinks to different sections?]
 
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                    <thead>
 
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                        <tr>
 
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                            <th> No. </th>
 
-
                            <th> ID </th>
 
-
                            <th> Name / Function </th>
 
-
                            <th> Source </th>
 
-
                            <th> State / Concentration / Date Made </th>
 
-
                            <th> Gene Size / Sequence </th>
 
-
                            <th> Initial Plasmid / Vector </th>
 
-
                            <th> Comments </th>
 
-
                        </tr>
 
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                    </thead>
 
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                    <tbody>
 
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                        <!--Lisbon plasmids-->
 
-
                        <tr> <!--(1) pAzoR / BBa_K1336000 REQUIRES OVERVIEW-->
 
-
                            <td> 1 </td>
 
-
                            <td> pAzoR / <a href="/Team:UCL/biobricks#BBa_K1336000">BBa_K1336000</a> </td>
 
-
                            <td> FMN-dependent NADH-azoreductase 1 </td>
 
-
                            <td> <em>Pseudomonas putida</em> </td>
 
-
                            <td> Miniprep,
 
-
                                <br>48 ng/uL,
 
-
                                <br>15/07/17, TO. </td>
 
-
                            <td> 597 bp <strong>[Check! Not 612 bp?]</strong>,
 
-
                                <br><a href=" http://www.ncbi.nlm.nih.gov/nuccore/26986745?report=fasta&from=3267527&to=3268138">Genomic Sequence</a> </td>
 
-
                            <td> Expression vector <a href="http://www.addgene.org/browse/sequence_vdb/2549/ ">pET-21a (+) <a href=" http://biochem.web.utah.edu/hill/links/pET21.pdf">(ampicillin resistant (ampR))</a>, initially cloned between <em>NdeI</em> and <em>BamHI</em>. </td>
 
-
                            <td> Plasmid provided by Lisbon; with <a href="http://www.ncbi.nlm.nih.gov/pubmed/21655981">Paper</a>. </td>
 
-
                        </tr>
 
-
                        <tr> <!--(2) p1B6 / BBa_K1336001-->
+
-
                            <td> 2 </td>
+
<h3>Experiment 1: Extraction of useful BioBrick plasmids from iGEM 2014 Distribution Kit - Date: [ddmmyy]</h3>
-
                            <td> p1B6 (AzoR 1B6) / <a href="/Team:UCL/biobricks#BBa_K1336001">BBa_K1336001</a> </td>
+
<div class="byline"><i class="icon-pencil"></i> Adam Denyer &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="Monday, October 15, 2013, 8:21 PM">October 15, 2013</abbr></div>
-
                            <td> Mutant: Heat-stable; FMN-dependent NADH-azoreductase 1 </td>
+
<p><strong>Protocols</strong><br/>  
-
                            <td> <em>Pseudomonas putida</em> </td>
+
<a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a>
-
                            <td> Miniprep,
+
<a href="/Team:UCL/protocols"><span class="label label-warning">transformation</span></a>
-
                                <br>68 ng/uL,
+
<a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a>
-
                                <br>15/07/14, TO.</td>
+
<a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a>
-
                            <td> 597 bp <strong>[Check! Not 612 bp?]</strong>,
+
<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p>
-
                                <br><a href="">Genomic Sequence</a><strong>[Add sequence! Sent from Lisbon, see DT]</strong> </td>
+
<p>senectus et netus et malesuada</p>
-
                            <td> Expression vector <a href="http://www.addgene.org/browse/sequence_vdb/2549/ ">pET-21a (+) <a href=" http://biochem.web.utah.edu/hill/links/pET21.pdf">(ampR)</a>, initially cloned between <em>NdeI</em> and <em>BamHI</em>. </td>
+
-
                            <td> Plasmid provided by Lisbon; with <a href=" http://www.ncbi.nlm.nih.gov/pubmed/24475252">Paper</a>. </td>
+
-
                        </tr>
+
-
                        <tr> <!--(3) pCotA / BBa_K1336002-->
+
-
                            <td> 3 </td>
+
-
                            <td> pCotA / <a href="/Team:UCL/biobricks#BBa_K1336002">BBa_K1336002</a> </td>
+
-
                            <td> Spore Coat Protein Laccase </td>
+
-
                            <td> <em>Bacillus subtilis</em> </td>
+
-
                            <td> Miniprep,  
+
-
                                <br>103 ng/uL,  
+
-
                                <br>15/07/14, TO. </td>
+
-
                            <td> 1733 bp <strong>[Check! Not 1539 bp?]</strong>
+
-
                                <br><a href="">Genomic Sequence</a><strong>[Add sequence! Sent from Lisbon, see DT]</strong> </td>
+
-
                            <td> Expression vector <a href="http://www.addgene.org/browse/sequence_vdb/2549/ ">pET-21a (+) <a href=" http://biochem.web.utah.edu/hill/links/pET21.pdf">(ampR)</a>, initially cloned between <strong><em>NheI</em></strong> and <em>BamHI</em>. </td>
+
-
                            <td> Plasmid provided by Lisbon; with <a href=" http://www.itqb.unl.pt/martins/index_files/JBC2002.pdf">Paper</a>. </td>
+
-
                        </tr>
+
-
                        <tr> <!--(4) pLiP / BBa_K1336003-->
+
-
                            <td> 4 </td>
+
-
                            <td> LiP / <a href="/Team:UCL/biobricks#BBa_K1336003">BBa_K1336003</a> </td>
+
-
                            <td> Lignin Peroxidase </td>
+
-
                            <td> <em>Phanerochaete chrysosporium</em> (White-Rot Fungi) </td>
+
-
                            <td> [Being synthesised by <a href="">Gene Oracle</a>],
+
-
                                <br>X ng/uL,
+
-
                                <br>dd/mm/yy, Gene Oracle. </td>
+
-
                            <td> X bp,
+
-
                                <br><a href="">Genomic Sequence</a> </td>
+
-
                            <td> [Cloned directly into expression vector, <a href="">pSB1C3</a>, between <em>EcoRI</em> and <em>PstI</em>.] </td>
+
-
                            <td> Synthesised (for free) by <a href="">Gene Oracle</a>. Sequence from <a href="">Paper</a>. </td>
+
-
                        </tr>
+
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                        <tr> <!--(5) pBsDyP / BBa_K1336004-->
+
-
                            <td> 5 </td>
+
-
                            <td> pBsDyP / <a href="/Team:UCL/biobricks#BBa_K1336004">BBa_K1336004</a> </td>
+
-
                            <td> Dye Decolourising Peroxidase BSU38260 </td>
+
-
                            <td> <em>Bacillus subtilis</em> </td>
+
-
                            <td> Miniprep,
+
-
                                <br>51 ng/uL,
+
-
                                <br>15/07/14, TO.</td>
+
-
                            <td> 1251 bp,
+
-
                                <br><a href="">Genomic Sequence</a><strong>[Add sequence! Sent from Lisbon, see DT]</strong> </td>
+
-
                            <td> Expression vector <a href="http://www.addgene.org/browse/sequence_vdb/2549/ ">pET-21a (+) <a href=" http://biochem.web.utah.edu/hill/links/pET21.pdf">(ampR)</a>, initially cloned between <em>NdeI</em> and <em>BamHI</em>. </td>
+
-
                            <td>Plasmid provided by Lisbon; with <a href="  http://www.ncbi.nlm.nih.gov/pubmed/23820555">Paper</a>. </td>
+
-
                        </tr>
+
-
                        <tr> <!--(6) pPpDyP / BBa_K1336005-->
+
-
                            <td> 6 </td>
+
-
                            <td> pPpDyP / <a href="/Team:UCL/biobricks#BBa_K1336005">BBa_K1336005</a> </td>
+
-
                            <td> Dye Decolourising Peroxidase PP_3248 </td>
+
-
                            <td> <em>Pseudomonas putida</em> </td>
+
-
                            <td> Miniprep,
+
-
                                <br>55 ng/uL,
+
-
                                <br>15/07/14, TO. </td>
+
-
                            <td> 861 bp <strong>[Check! Not 864 bp?]</strong>,
+
-
                                <br><a href="">Genomic Sequence</a><strong>[Add sequence! Sent from Lisbon, see DT]</strong> </td>
+
-
                            <td> Expression vector <a href="http://www.addgene.org/browse/sequence_vdb/2549/ ">pET-21a (+) <a href=" http://biochem.web.utah.edu/hill/links/pET21.pdf">(ampR)</a>, initially cloned between <em>NdeI</em> and <em>BamHI</em>. </td>
+
-
                            <td>Plasmid provided by Lisbon; with <a href="  http://www.ncbi.nlm.nih.gov/pubmed/23820555">Paper</a>. </td>
+
-
                        </tr>
+
-
                        <!--Distribution BioBricks; PAGES TO BE MADE!-->
+
<h3>Experiment 2: Transforming <i>E. coli</i> with Azo-reductase plasmids - Date: [ddmmyy]</h3>
-
                        <tr> <!--(7) BBa_J04450-->
+
<p><strong>Protocols</strong><br/>  
-
                            <td> 7 </td>
+
<a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a>
-
                            <td> <a href="http://parts.igem.org/Part:BBa_J04450">BBa_J04450</a> </td>
+
<a href="/Team:UCL/protocols"><span class="label label-warning">transformation</span></a>
-
                            <td> RFP Coding Device</td>
+
<a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a>
-
                            <td> Spring 2014 BioBrick Distribution.
+
<a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a>
-
                            <br><a href="http://parts.igem.org/partsdb/get_part.cgi?part=BBa_J04450">Plate 4, Well 4B</a>. <strong>[Check! DT?]</strong>. </td>
+
<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p>
-
                            <td> (1) Miniprep,
+
<p>senectus et netus et malesuada</p>
-
                                <br>333 ng/uL,
+
 
-
                                <br>01/07/14, TO.
+
<h3>Experiment 3: Diagnostic digest of azo-reductase plasmids - Date: [ddmmyy]</h3>
-
                                <br>
+
<p><strong>Protocols</strong><br/>  
-
                                <br>(2) Miniprep,
+
<a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a>
-
                                <br>38 ng/uL (NanoDrop, dodgy!)
+
<a href="/Team:UCL/protocols"><span class="label label-warning">transformation</span></a>
-
                                <br>01/07/14, TO. </td>
+
<a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a>
-
                            <td> <a href="http://parts.igem.org/cgi/sequencing/one_blast.cgi?id=21336">1069 bp</a>,
+
<a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a>
-
                                <br><a href="">Genomic Sequence</a><strong>[Add sequence! Made from combined BioBricks?]</strong> </td>
+
<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p>
-
                            <td> Plasmid Backbone: <a href="">pSB1C3</a>, i.e. chloramphenicol resistant (camR). </td>
+
<p>senectus et netus et malesuada</p>
-
                            <td> LacI-, and CAP-, sensitive; can fail if system contains LacI or CAP protein!
+
 
-
                            <br>RFP Coding Device contains: LacI (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0010">R0010</a>), strong RBS (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_B0034">B0034</a>), mRFP1 (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_E1010">E1010</a>), and double terminator (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_B0015">B0015</a> = <a href="http://parts.igem.org/wiki/index.php/Part:BBa_B0010">B0010</a>+<a href="http://parts.igem.org/wiki/index.php/Part:BBa_B0012">B0012</a>).</td>
+
<h3>Experiment 4: Creation of azo-reductase BioBrick parts from plasmids - Date: [ddmmyy]</h3>
-
                        </tr>
+
<p><strong>Protocols</strong><br/>  
-
                        <tr> <!--(8) BBa_R0010-->
+
<a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a>
-
                            <td> 8 </td>
+
<a href="/Team:UCL/protocols"><span class="label label-warning">transformation</span></a>
-
                            <td> <a href="http://parts.igem.org/Part:BBa_R0010">BBa_R0010</a> </td>
+
<a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a>
-
                            <td> Promoter (LacI regulated)</td>
+
<a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a>
-
                            <td> Spring 2014 BioBrick Distribution.
+
<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p>
-
                            <br><a href="http://parts.igem.org/partsdb/get_part.cgi?part=BBa_R0010">Plate 3, Well 4G</a>. <strong>[Check! DT?]</strong>. </td>
+
<p>senectus et netus et malesuada</p>
-
                            <td> Miniprep,
+
 
-
                                <br>329.1 ng/uL,
+
<h3>Experiment 5: Diagnostic digest of azo-reductase BioBrick parts - Date: [ddmmyy]</h3>
-
                                <br>01/07/14, TO. </td>
+
<p><strong>Protocols</strong><br/>  
-
                            <td> 200 bp,
+
<a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a>
-
                                <br><a href="">Genomic Sequence</a><strong>[Add sequence!]</strong> </td>
+
<a href="/Team:UCL/protocols"><span class="label label-warning">transformation</span></a>
-
                            <td> Plasmid Backbone: <a href="">pSB1C3</a>, i.e. chloramphenicol resistant (camR).
+
<a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a>
-
                                <br><a href="http://beta.labgeni.us/registries/parts_registry/?part=BBa_R0010">Plasmid / Vector Map</a>. </td>
+
<a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a>
-
                            <td> This part is an inverting regulator sensitive to LacI and CAP. In the absence of LacI and CAP proteins, this part promotes transcription; in their presence, the part inhibits transcription. LacI can be inhibited by <a href="openwetware.org/wiki/IPTG">IPTG</a>.</td>
+
<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p>
-
                        </tr>
+
<p>senectus et netus et malesuada</p>
-
                        <tr> <!--(9) BBa_R0011-->
+
 
-
                            <td> 9 </td>
+
<h3>Experiment 6: Assembling azo-reductase BioBrick Device(s) - Date: [ddmmyy]</h3>
-
                            <td> <a href="http://parts.igem.org/Part:BBa_R0011">BBa_R0011</a> </td>
+
<p><strong>Protocols</strong><br/>  
-
                            <td> Promoter (LacI regulated, lambda pL hybrid)</td>
+
<a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a>
-
                            <td> Spring 2014 BioBrick Distribution.
+
<a href="/Team:UCL/protocols"><span class="label label-warning">transformation</span></a>
-
                            <br><a href="http://parts.igem.org/partsdb/get_part.cgi?part=BBa_R0011">Plate 2, Well 6D</a> (Inconsistent sequencing!). <strong>[Check! DT? / Maybe use Spring 2013 Distribution, Plate 5, Well 6G.]</strong>. </td>
+
<a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a>
-
                            <td> Miniprep,
+
<a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a>
-
                                <br>38 ng/uL (NanoDrop, dodgy!),
+
<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p>
-
                                <br>01/07/14, TO. </td>
+
<p>senectus et netus et malesuada</p>
-
                            <td> 55 bp,
+
 
-
                                <br><a href="">Genomic Sequence</a><strong>[Add sequence!]</strong> </td>
+
<h3>Experiment 7: Characterisation of azo-reductase BioBrick devices - Date: [ddmmyy]</h3>
-
                            <td> Plasmid Backbone: <a href="">pSB1C3</a>, i.e. chloramphenicol resistant (camR).
+
<p><strong>Protocols</strong><br/>  
-
                                <br><a href="http://beta.labgeni.us/registries/parts_registry/?part=BBa_R0011">Plasmid / Vector Map</a>. </td>
+
<a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a>
-
                            <td> Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1.</td>
+
<a href="/Team:UCL/protocols"><span class="label label-warning">transformation</span></a>
-
                        </tr>
+
<a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a>
-
                        <tr> <!--(10) BBa_K314103-->
+
<a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a>
-
                            <td> 10 </td>
+
<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p>
-
                            <td> <a href="http://parts.igem.org/Part:BBa_K314103">BBa_K314103</a> </td>
+
<p>senectus et netus et malesuada</p>
-
                            <td> Lac induced expression cassette </td>
+
-
                            <td> Spring 2014 BioBrick Distribution.
+
-
                            <br><a href="http://parts.igem.org/partsdb/get_part.cgi?part=BBa_K314103">Plate 1, Well 4D</a>. <strong>[Check! DT?]</strong>. </td>
+
-
                            <td> Miniprep,
+
-
                                <br>334 ng/uL,
+
-
                                <br>01/07/14, TO. </td>
+
-
                            <td> 1638 bp,
+
-
                                <br><a href="">Genomic Sequence</a><strong>[Add sequence!]</strong> </td>
+
-
                            <td> Plasmid Backbone: <a href="">pSB1C3</a>, i.e. chloramphenicol resistant (camR).
+
-
                                <br><a href="http://beta.labgeni.us/registries/parts_registry/?part=BBa_K314103">Plasmid / Vector Map</a>. </td>
+
-
                            <td> Lactose (<a href="openwetware.org/wiki/IPTG">IPTG</a>) inducible protein expression insert includes f1 origin (<a href="http://parts.igem.org/Part:BBa_K314110">K314110</a>), a Lac I generator (<a href="http://parts.igem.org/Part:BBa_K314111">K314111</a>), a lactose inducible promoter (<a href="http://parts.igem.org/Part:BBa_R0011">R0011</a>), and the Elowitz standard RBS (<a href="http://parts.igem.org/Part:BBa_B0034">B0034</a>).</td>
+
-
                        </tr>
+
-
                        <tr> <!--(11) BBa_K206000-->
+
-
                            <td> 11 </td>
+
-
                            <td> <a href="http://parts.igem.org/Part:BBa_K206000">BBa_K206000</a> </td>
+
-
                            <td> pBAD Strong Promoter </td>
+
-
                            <td> Spring 2014 BioBrick Distribution.
+
-
                            <br><a href="http://parts.igem.org/partsdb/get_part.cgi?part=BBa_K206000">Plate 3, Well 14A</a>. <strong>[Check! DT?]</strong>. </td>
+
-
                            <td> Miniprep,
+
-
                                <br>144 ng/uL,
+
-
                                <br>01/07/14, TO. </td>
+
-
                            <td> 130 bp,
+
-
                                <br><a href="">Genomic Sequence</a><strong>[Add sequence!]</strong> </td>
+
-
                            <td> Plasmid Backbone: <a href="">pSB1C3</a>, i.e. chloramphenicol resistant (camR).
+
-
                                <br><a href="http://beta.labgeni.us/registries/parts_registry/?part=BBa_K206000">Plasmid / Vector Map</a> </td>
+
-
                            <td> pBAD is an <em>E. coli</em> promoter that is induced by L-arabinose. In the absence of arabinose, the repressor protein AraC (<a href="http://parts.igem.org/Part:I13458">BBa_I13458</a>) binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription; in its presence, transcription is permitted.</td>
+
-
                        </tr>
+
-
                        <tr> <!--(12) BBa_B0034-->
+
-
                            <td> 12 </td>
+
-
                            <td> <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a> </td>
+
-
                            <td> RBS </td>
+
-
                            <td> Spring 2014 BioBrick Distribution.
+
-
                            <br><a href="http://parts.igem.org/partsdb/get_part.cgi?part=BBa_B0034">Plate 4, Well 1N</a>. <strong>[Check! DT?]</strong>. </td>
+
-
                            <td> Miniprep,
+
-
                                <br>156.5 ng/uL,
+
-
                                <br>01/07/14, TO. </td>
+
-
                            <td> 12 bp,
+
-
                                <br><a href="">Genomic Sequence</a><strong>[Add sequence!]</strong> </td>
+
-
                            <td> Plasmid Backbone: <strong><a href="">pSB1A2</a></strong>, i.e. ampicillin resistant (ampR).
+
-
                                <br><a href="http://beta.labgeni.us/registries/parts_registry/?part=BBa_B0034">Plasmid / Vector Map</a> </td>
+
-
                            <td>RBS based on Elowitz (1999) repressilator.</td>
+
-
                        </tr>
+
-
                        <tr> <!--(13) BBa_K518012-->
+
-
                            <td> 13 </td>
+
-
                            <td> <a href="http://parts.igem.org/Part:BBa_K518012">BBa_K518012</a> </td>
+
-
                            <td> RBS + RFP + double Terminator </td>
+
-
                            <td> Spring 2014 BioBrick Distribution.
+
-
                            <br><a href="http://parts.igem.org/partsdb/get_part.cgi?part=BBa_K518012">Plate 1, Well 18C</a>. <strong>[Check! DT?]</strong>. </td>
+
-
                            <td> (1) Miniprep,
+
-
                                <br>49 ng/uL,
+
-
                                <br>01/07/14, TO.
+
-
                                <br>
+
-
                                <br>(2) Miniprep,
+
-
                                <br> 219.2 ng/uL,
+
-
                                <br> 08/08/14, YKH. </td>
+
-
                            <td> 828 bp,
+
-
                                <br><a href="">Genomic Sequence</a><strong>[Add sequence!]</strong> </td>
+
-
                            <td> Plasmid Backbone: <a href="">pSB1C3</a>, i.e. chloramphenicol resistant (camR).
+
-
                                <br><a href="http://beta.labgeni.us/registries/parts_registry/?part=BBa_K518012">Plasmid / Vector Map</a> </td>
+
-
                            <td> This Coding Device contains: RBS.3 (medium) (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_B0032">B0032</a>), mRFP1 (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_E1010">E1010</a>), and double terminator (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_B0014">B0014</a> = <a href="http://parts.igem.org/wiki/index.php/Part:BBa_B0012">B0012</a>+<a href="http://parts.igem.org/wiki/index.php/Part:BBa_B0011">B0011</a>).</td>
+
-
                        </tr>
+
-
                        <tr> <!--(14) BBa_B0012-->
+
-
                            <td> 14 </td>
+
-
                            <td> <strong>[CHECK: BAD PART !]</strong>
+
-
                            <br><a href="http://parts.igem.org/Part:BBa_B0012">BBa_B0012 (2)</a> </td>
+
-
                            <td> Transcription Terminator for <em>E. coli</em> RNA polymerase </td>
+
-
                            <td> Spring 2014 BioBrick Distribution.
+
-
                            <br><a href="http://parts.igem.org/partsdb/get_part.cgi?part=BBa_K518012">Plate 2, Well 2B</a>. <strong>[Check! DT?]</strong>. </td>
+
-
                            <td> Miniprep,
+
-
                                <br>128 ng/uL,
+
-
                                <br>01/07/14, TO. </td>
+
-
                            <td> 41 bp,
+
-
                                <br><a href="">Genomic Sequence</a><strong>[Add sequence!]</strong> </td>
+
-
                            <td> Plasmid Backbone: <a href="">pSB1C3</a>, i.e. chloramphenicol resistant (camR).
+
-
                                <br><a href="http://beta.labgeni.us/registries/parts_registry/?part=BBa_B0012">Plasmid / Vector Map</a> </td>
+
-
                            <td>TE from coliphage T7. <strong>This is a bad terminator (Experience: Fails)</strong>. It is a promoter in the reverse direction.</td>
+
-
                        </tr>
+
-
                        <tr> <!--(#) p-->
+
-
                            <td> # </td>
+
-
                            <td> ID </td>
+
-
                            <td> Name / Function </td>
+
-
                            <td> Source </td>
+
-
                            <td> State / Concentration / Date Made </td>
+
-
                            <td> Gene Size / Sequence </td>
+
-
                            <td> Initial Plasmid / Vector </td>
+
-
                            <td> Comments </td>
+
-
                        </tr>
+
-
                    </tbody>
+
-
                    </table>
+
-
                <p><strong>Pellentesque habitant morbi tristique</strong> senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. <em>Aenean ultricies mi vitae est.</em> Mauris placerat eleifend leo. Quisque sit amet est et sapien ullamcorper pharetra. Vestibulum erat wisi, condimentum sed, <code>commodo vitae</code>, ornare sit amet, wisi. Aenean fermentum, elit eget tincidunt condimentum, eros ipsum rutrum orci, sagittis tempus lacus enim ac dui. <a href="#">Donec non enim</a> in turpis pulvinar facilisis. Ut felis.</p>
 
-
                <p>Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. <em>Aenean ultricies mi vitae est.</em> Mauris placerat eleifend leo. Quisque sit amet est et sapien ullamcorper pharetra. Vestibulum erat wisi, condimentum sed, <code>commodo vitae</code>, ornare sit amet, wisi. Aenean fermentum, elit eget tincidunt condimentum, eros ipsum rutrum orci, sagittis tempus lacus enim ac dui. <a href="#">Donec non enim</a> in turpis pulvinar facilisis. Ut felis.</p>
 
-
                <p>Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. <em>Aenean ultricies mi vitae est.</em> Mauris placerat eleifend leo. Quisque sit amet est et sapien ullamcorper pharetra. Vestibulum erat wisi, condimentum sed, <code>commodo vitae</code>, ornare sit amet, wisi. Aenean fermentum, elit eget tincidunt condimentum, eros ipsum rutrum orci, sagittis tempus lacus enim ac dui. <a href="#">Donec non enim</a> in turpis pulvinar facilisis. Ut felis.</p>
 
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Revision as of 13:24, 5 September 2014

Goodbye Azodye UCL iGEM 2014

Experiments

Laboratory Team

List of Experiments

  1. Experiment 01: Extraction of useful BioBrick plasmids from iGEM 2014 Distribution Kit
  2. Experiment 02: Transforming E. coli with Azo-reductase plasmids
  3. Experiment 03: Diagnostic digest of azo-reductase plasmids
  4. Experiment 04: Creation of azo-reductase BioBrick parts from plasmids
  5. Experiment 05: Diagnostic digest of azo-reductase BioBrick parts
  6. Experiment 06: Assembling azo-reductase BioBrick Device(s)
  7. Experiment 07: Characterisation of azo-reductase BioBrick devices

Experiment 1: Extraction of useful BioBrick plasmids from iGEM 2014 Distribution Kit - Date: [ddmmyy]

Adam Denyer    October 15, 2013

Protocols
competent cells transformation miniprep digest gel

senectus et netus et malesuada

Experiment 2: Transforming E. coli with Azo-reductase plasmids - Date: [ddmmyy]

Protocols
competent cells transformation miniprep digest gel

senectus et netus et malesuada

Experiment 3: Diagnostic digest of azo-reductase plasmids - Date: [ddmmyy]

Protocols
competent cells transformation miniprep digest gel

senectus et netus et malesuada

Experiment 4: Creation of azo-reductase BioBrick parts from plasmids - Date: [ddmmyy]

Protocols
competent cells transformation miniprep digest gel

senectus et netus et malesuada

Experiment 5: Diagnostic digest of azo-reductase BioBrick parts - Date: [ddmmyy]

Protocols
competent cells transformation miniprep digest gel

senectus et netus et malesuada

Experiment 6: Assembling azo-reductase BioBrick Device(s) - Date: [ddmmyy]

Protocols
competent cells transformation miniprep digest gel

senectus et netus et malesuada

Experiment 7: Characterisation of azo-reductase BioBrick devices - Date: [ddmmyy]

Protocols
competent cells transformation miniprep digest gel

senectus et netus et malesuada

Contact Us

University College London
Gower Street - London
WC1E 6BT
Biochemical Engineering Department
Phone: +44 (0)20 7679 2000
Email: ucligem2014@gmail.com

Follow Us

Retrieved from "http://2014.igem.org/Team:UCL/Science/Experiment"