Team:TU Eindhoven

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Revision as of 19:31, 2 September 2014

TU Eindhoven iGEM Team

TU Eindhoven iGEM 2014 Project

Our goal this year in the iGEM competition is to create a new tool for the synthetic biologist’s toolbox; a tool that allows genetically engineered machines to operate inside the human body without causing an immune response. We want to achieve this by combining polymer chemistry and microfluidics to create individually encapsulated bacteria. Want to learn more? Go to our Project Overview!

Microfluidics

We are busy optimizing our droplet device to get the right parameters for our final device. The design for this device is also being finalized. The device will be used to create droplets with a few bacteria inside in which the polymer chemistry will be done. After the reaction we will test the formation of the encapsulation with FACS. For more information on the subject of Microfluidics, check our Microfluidics Page!

Lab Work

Currently we are making our designed plasmids, after the sequencing results we will hopefully be able to start with protein expression and turning our designed genes into biobricks! See the Lab Work Page for more information about our achievements in the lab!

Modeling

We are currently busy researching possible modelling projects, come back later for more information!

Policy & Practices

For Policy & Practices two big projects are currently in focus: creating a picture book about lab work for our younger audience, and building a game about synthetic biology for middle school students.

Team News

Microfluidics: Tests with our cell encapsulation devices are currently performed with use of fluorescent beads as a replacement of bacterial cells.

  • 30 August 2014

Microfluidics: Testing with polyacrylamide droplets is going well.

  • 31 July 2014


Lab Work: Click reaction results show that the product forms, optimization of the reaction is now underway.

  • 29 July 2014

    • Lab Work: FACS analysis using fluorescent DBCO molecules shows that our modified protein is expressed on the membrane, we are now testing with DBCO molecules with PEG tails.

    • 25 July 2014

Contact


Eindhoven University of Technology
Den Dolech 2, 5612 AZ Eindhoven
The Netherlands
Ceres 0.04

iGEM@tue.nl

+31 40 247 55 59

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Created by iGEM Team TU Eindhoven 2014

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