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Team:Bielefeld-CeBiTec/Notebook/Journal/CO2-fixation/Sep
From 2014.igem.org
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| + | <ul> <!-- csoS1-4 für GFP Fusion --> | ||
| + | <li><i><b>csoS1-4</b></i></li> | ||
| + | <ul> | ||
| + | <li>We tried to amplify <i>csoS1-4</i> (shell proteins) for a fusion with GFP.</li> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PCR" target="_blank">PCR amplification</a> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw-GFP-csoS1B" target="_blank">fw-GFP-csoS1B</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rv-GFP-csoS1A" target="_blank">rv-rv-GFP-csoS1A</a>)</li> | ||
| + | <ul> | ||
| + | <li>Annealing temperature: 54 °C</li> | ||
| + | <li>Bands as expected (~4000 bp (~1800 bp <i>csoS1-4</i>, ~2200 bp pSB1C3))</li> | ||
| + | </ul> | ||
| + | <li>PCR products were <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#DNAPurificationbyCentrifugation" target="_blank">purified</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | |||
| + | <br> | ||
| + | |||
| + | <ul> <!-- csoS1-4 Fusion mit can --> | ||
| + | <li><b><i>can</i> and <i>csoS1-4</i></b></li> | ||
| + | <ul> | ||
| + | <li>This week we tried to find positive clones of our transformation.</li> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#ColonyPCR" target="_blank">Colony PCR</a> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#VF-Primer" target="_blank">VF-Primer</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#VR-Primer" target="_blank">VR-Primer</a>) | ||
| + | </li> | ||
| + | <ul> | ||
| + | <li>Annealing temperature: 55 °C</li> | ||
| + | <li>Bands as expected (~3600 bp)</li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of pSB1C3_can_csoS1-4</li> | ||
| + | </ul> | ||
| + | |||
| + | |||
| + | </ul> | ||
| + | </ul> | ||
| + | |||
| + | |||
| + | |||
| + | |||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 21:02, 26 September 2014
 |
September |
 |
- csoS1-4
- We tried to amplify csoS1-4 (shell proteins) for a fusion with GFP.
- PCR amplification (fw-GFP-csoS1B, rv-rv-GFP-csoS1A)
- Annealing temperature: 54 °C
- Bands as expected (~4000 bp (~1800 bp csoS1-4, ~2200 bp pSB1C3))
- PCR products were purified
- can and csoS1-4
- This week we tried to find positive clones of our transformation.
- Colony PCR (VF-Primer, VR-Primer)
- Annealing temperature: 55 °C
- Bands as expected (~3600 bp)
- Plasmid isolation of pSB1C3_can_csoS1-4
