Team:Evry/Notebook/Sensing/PCBs/week6

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<h2> Sensor construction hbpR-hbpC </h2>  
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<h5> Sensor construction hbpR-hbpC </h5>  
<u>Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:</u>
<u>Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:</u>
<br/> Plate on LB-Agar-Amp (25mL LB-Agar + 25 µL Amp) and incubation overnight at 37°C
<br/> Plate on LB-Agar-Amp (25mL LB-Agar + 25 µL Amp) and incubation overnight at 37°C
<br/> Culturing of 1 colony in 5mL of LB + 5µL Amp and incubation at 37°C, 200rpm
<br/> Culturing of 1 colony in 5mL of LB + 5µL Amp and incubation at 37°C, 200rpm
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</p>
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Revision as of 16:42, 18 August 2014

Week 6

Sensor construction hbpR-hbpC
Extraction hbpR sequence and hbpC promoter sequence from pHYB109 plasmid in DH5a:
Plate on LB-Agar-Amp (25mL LB-Agar + 25 µL Amp) and incubation overnight at 37°C
Culturing of 1 colony in 5mL of LB + 5µL Amp and incubation at 37°C, 200rpm

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