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Team:Cambridge-JIC/Constructs/35S-HBRmRFP
From 2014.igem.org
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<li><a href="https://docs.google.com/spreadsheets/d/1RwA7YHMnZ4a0M67RMbegWU5ee3MJRo62qOcBJj537yg/edit#gid=1660015451">PCR Spreadsheet (on google drive)</a></li> | <li><a href="https://docs.google.com/spreadsheets/d/1RwA7YHMnZ4a0M67RMbegWU5ee3MJRo62qOcBJj537yg/edit#gid=1660015451">PCR Spreadsheet (on google drive)</a></li> | ||
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Latest revision as of 13:53, 31 July 2014
35S-H2BmRFP
Design
Aim of the construct
- This is a simple construct for me (Hugh) to check my practical skills. Apparently it has worked for others in the past.
End goal plasmid
Experiments (to date)
PCR
Attempt 1:- Start date/time: 30/07, morning
- End date/time:30/07, morning
- UIDs of primers/plasmids used:
- A1(DEXX3), P42(35S-H2BF), P41(nosT-mRFP-B)
- B14(35S-rLTI), P17(P1_B), P15(nosT-F)
- B14(35S-rLTI), P16(P1_F), P14(35S-B)
- PCR Spreadsheet (on google drive)
Gibson
Attempt 1:- Start date/time:
- End date/time:
- Comments:
E-Coli transformation
Attempt 1:- Start date/time:
- End date/time:
- Comments:
Agrobacteria transformation
Attempt 1:- Start date/time:
- End date/time:
- Induce comments:
- Growth comments:
- Electroporation/selection comments:
Spore preparation
Attempt 1:- Start date/time:
- End date/time:
- Comments:
Spore transformation
Attempt 1:- Start date/time:
- End date/time:
- Comments:
Evaluation
- Start date/time:
- End date/time:
- Comments:
