Team:Cambridge-JIC/Constructs/35S-HBRmRFP

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35S-H2BmRFP

Design

Aim of the construct

This is a simple construct for me (Hugh) to check my practical skills. Apparently it has worked for others in the past.

End goal plasmid

Experiments (to date)

PCR

Attempt 1:

Start date/time: 30/07, morning
End date/time:30/07, morning
UIDs of primers/plasmids used:
- A1(DEXX3), P42(35S-H2BF), P41(nosT-mRFP-B)
- B14(35S-rLTI), P17(P1_B), P15(nosT-F)
- B14(35S-rLTI), P16(P1_F), P14(35S-B)
PCR Spreadsheet (on google drive)

Gibson

Attempt 1:

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E-Coli transformation

Attempt 1:

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Agrobacteria transformation

Attempt 1:

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Induce comments:
Growth comments:
Electroporation/selection comments:

Spore preparation

Attempt 1:

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Spore transformation

Attempt 1:

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Evaluation

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