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Team:Cambridge-JIC/Constructs/35S-HBRmRFP
From 2014.igem.org
(Difference between revisions)
| Line 27: | Line 27: | ||
Attempt 1: | Attempt 1: | ||
<ul> | <ul> | ||
| - | <li>Start date/time: | + | <li>Start date/time: 30/07, morning</li> |
| - | <li>End date/time: | + | <li>End date/time:30/07, morning</li> |
<li> UIDs of primers/plasmids used: | <li> UIDs of primers/plasmids used: | ||
<ul> | <ul> | ||
| - | <li> A1, | + | <li> A1(DEXX3), P42(35S-H2BF), P41(nosT-mRFP-B)</li> |
| - | <li> | + | <li> B14(35S-rLTI), P17(P1_B), P15(nosT-F)</li> |
| - | <li> | + | <li> B14(35S-rLTI), P16(P1_F), P14(35S-B)</li> |
| - | + | </ul></li> | |
<li><a href="https://docs.google.com/spreadsheets/d/1RwA7YHMnZ4a0M67RMbegWU5ee3MJRo62qOcBJj537yg/edit#gid=1660015451">PCR Spreadsheet (on google drive)</a></li> | <li><a href="https://docs.google.com/spreadsheets/d/1RwA7YHMnZ4a0M67RMbegWU5ee3MJRo62qOcBJj537yg/edit#gid=1660015451">PCR Spreadsheet (on google drive)</a></li> | ||
Revision as of 11:40, 31 July 2014
35S-H2BmRFP
Design
Aim of the construct
- This is a simple construct for me (Hugh) to check my practical skills. Apparently it has worked for others in the past.
End goal plasmid
Experiments (to date)
PCR
Attempt 1:- Start date/time: 30/07, morning
- End date/time:30/07, morning
- UIDs of primers/plasmids used:
- A1(DEXX3), P42(35S-H2BF), P41(nosT-mRFP-B)
- B14(35S-rLTI), P17(P1_B), P15(nosT-F)
- B14(35S-rLTI), P16(P1_F), P14(35S-B)
- PCR Spreadsheet (on google drive)
- Gel 27/07/2014
Gibson
Attempt 1:- Start date/time:
- End date/time:
- Comments:
E-Coli transformation
Attempt 1:- Start date/time:
- End date/time:
- Comments:
Agrobacteria transformation
Attempt 1:- Start date/time:
- End date/time:
- Induce comments:
- Growth comments:
- Electroporation/selection comments:
Spore preparation
Attempt 1:- Start date/time:
- End date/time:
- Comments:
Spore transformation
Attempt 1:- Start date/time:
- End date/time:
- Comments:
Evaluation
- Start date/time:
- End date/time:
- Comments:
