Team:Cambridge-JIC/Constructs/35S-HBRmRFP

From 2014.igem.org

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<h1> Gal4 - Hap1GR - H2BmRFP</h1>
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<h1> 35S-H2BmRFP</h1>
<h2> Design </h2>
<h2> Design </h2>
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<h4> Aim of the construct </h4>
<h4> Aim of the construct </h4>
<ul>  
<ul>  
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<li> We'll try out 35s->GAL4->HAP1GR->H2BmRFP. </li>
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<li> </li>
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<li> Since H2BmRFP has not been tried in Mp, we'll also try 35s->H2BmRFP. A positive control for lab technique will be 35s->Venus N7</li>
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<li> We'll also use FF's original plasmid to try 35s-Hap1GR-mRFP, as a control for the Hap1GR. I expect low transformation rate as in the original plasmid, we're using 35s as promoter for HygR selection marker.
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<li> Not sure whether 35s->Gal4 has been tried (check with BP), so 35s-Gal4-venus n7 is doable with BP's construct as a control </li>
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<li> As another control, we'll try 35s-Gal4-H2mRFP </li>
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</ul>
</ul>
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</div>
</div>
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<h4> End goal plasmid </h4>
<h4> End goal plasmid </h4>
<ul>  
<ul>  
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<li> <img src="https://static.igem.org/mediawiki/2014/f/fd/Cambridge-JIC_constructs_GAL4-HAP1GR-H2mRFP.png" width = "100%"> </li>
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<li> <!--<img>--> </li>
</ul>
</ul>
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</div>
</div>
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<h2> Experimentation (up to date)</h2>
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<h2> Experiments (to date)</h2>
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<div id="PCR" align="left">
<div id="PCR" align="left">
<h4>PCR</h4>
<h4>PCR</h4>
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</ul>
</ul>
</div>
</div>
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<div id="Spore preparation" align="left">
<div id="Spore preparation" align="left">
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<h4>Spore preparation</h4>
<h4>Spore preparation</h4>
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Attempt 1:
Attempt 1:
<ul>
<ul>
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<div id="Spore transformation" align="left">
<div id="Spore transformation" align="left">
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<h4> Spore transformation</h4>
<h4> Spore transformation</h4>
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Attempt 1:
Attempt 1:
<ul>
<ul>
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<div id="Evaluation" align="left">
<div id="Evaluation" align="left">
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<h2> Evaluation</h2>
<h2> Evaluation</h2>
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<ul>
<ul>
<li>Start date/time:</li>
<li>Start date/time:</li>

Revision as of 17:22, 30 July 2014