Team:Paris Saclay/Notebook/July/10

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(Difference between revisions)
(→Thursday 10th July)
(→Thursday 10th July)
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Negative control groups:
Negative control groups:
*pET28a: without plasmid, nothing grows.
*pET28a: without plasmid, nothing grows.
-
*J23100, J23106, J23114: without the BioBrick, nothing grows.
+
*BBa_J23100, BBa_J23106, BBa_J23114: without the BioBrick, nothing grows.
'''Results:'''
'''Results:'''
*pET28a: Nothing grows with the strains DH5α, MG1655, MG1655Z1. But the strain DH5α+ grows well.
*pET28a: Nothing grows with the strains DH5α, MG1655, MG1655Z1. But the strain DH5α+ grows well.
-
*J23100, J23106, J23114: The strain DH5α+ grow well and the 3 others strains grow less. They have to be more concentrated.
+
*BBa_J23100, BBa_J23106, BBa_J23114: The strain DH5α+ grow well and the 3 others strains grow less. They have to be more concentrated.
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]

Revision as of 13:04, 24 July 2014

Contents

Thursday 10th July

Lab work

1 - Plasmid DNA Extraction:

by Romain and Sean

Plasmids used:

  • BBa_B0015, dble ter
  • FNR_RBS_amilCP-Ter DH5αZ1

Protocol

2 - Transformation of competent cells E.coli

by Fabio and Maher

Competent strains used:

  • DH5α
  • MG1655
  • MG1655Z1
  • DH5α+ (given by Solenne)

(The strains DH5α, MG1655 and MG1655Z1 were prepared by Mathias and Mathieu)

BioBricks used:

  • BBa_J23100 (Amp R)(Dilutions: Concentrated, 0 and 10-1)
  • BBa_J23106 (Amp R)(Dilutions: Concentrated, 0 and 10-1)
  • BBa_J23114 (Amp R)(Dilutions: Concentrated, 0 and 10-1)

+ pET28a (Kan R)(Dilutions: 0, 10-1 and 10-2) (pET28a is a plasmid used to verify that the 4 strains are competent).

Negative control groups:

  • pET28a: without plasmid, nothing grows.
  • BBa_J23100, BBa_J23106, BBa_J23114: without the BioBrick, nothing grows.

Results:

  • pET28a: Nothing grows with the strains DH5α, MG1655, MG1655Z1. But the strain DH5α+ grows well.
  • BBa_J23100, BBa_J23106, BBa_J23114: The strain DH5α+ grow well and the 3 others strains grow less. They have to be more concentrated.

Protocol

Back to the calendar

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