Team:NTU Taida/M5

(1). adjustment of TE value : Findings: TE value is the absolute rather than the relative value of rbs. Ajusting TE value can alter the magnitude of concentration. (comparison with standard)

(2). Adjustment of degration rate of mRNA and Protein : Findings: Ajusting degration rate can alter the time scale. (comparison with standard) We illustrate the result by showing different combinations of adjusting degration rate of mRNA and protein. ❖ altering protein degration rate

❖ altering mRNA degration rate

❖ altering both protein and mRNA degration rate

(3). adjustment of promoter PfadBA strength: Findings: Increasing PfadBA streingth will increase concentration of CI and decrease the concentration of GFP. (comparison with standard)

(4). adjustment of the concentration of ayl-CoA: Findings: decreasing the concentration of acyl-coA, the concentration of CI will decrease and GFP will increase. (comparison with standard)

(5). Overexpression of FadL and FadR : Findings: Thereotically, when we overexpression the FadL and FadR (under non-overexpression of acyl-coA), the concentration of CI must decrease due to the competition between acyl-coA and FadR. However, the result remain the same. We assume that it might be our limitation of curve fitting due to the lack of experimental data. And thus we aren’t able to use the whole differential functions(see the parameter setting and initial conditions above)