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Team:UC Davis/Signal Oil
From 2014.igem.org
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<p>With a working model, it was time for the ultimate test: Olive Oil<br><br> | <p>With a working model, it was time for the ultimate test: Olive Oil<br><br> | ||
| - | Nine samples of Extra Virgin Olive Oil were obtained and <a href="https://2014.igem.org/Team:UC_Davis/Protein_Engineering_Test" class="brightlink">prepared</a> for assay. The velocities were recorded with each enzyme for a total of 27 data points. We used the best catalytic matrix from our previous model and again inverted the matrix and multiplied by the observed velocity. The results are below:<br> | + | Nine samples of Extra Virgin Olive Oil were obtained and <a href="https://2014.igem.org/Team:UC_Davis/Protein_Engineering_Test" class="brightlink">prepared</a> for assay where three of the samples were known to be rancid. The velocities were recorded with each enzyme for a total of 27 data points. We used the best catalytic matrix from our previous model and again inverted the matrix and multiplied by the observed velocity. The results are below:<br> |
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<p align="center"><img src="https://static.igem.org/mediawiki/2014/a/a9/OliveOil.png" style="margin-left:auto;margin-right:auto;border:1.5px solid #212f20;"/></p><br> | <p align="center"><img src="https://static.igem.org/mediawiki/2014/a/a9/OliveOil.png" style="margin-left:auto;margin-right:auto;border:1.5px solid #212f20;"/></p><br> | ||
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| + | We noticed to outliers based on the unsaturated aldehyde prediction. </p> | ||
<div style="margin:auto;display:block;float:center"> | <div style="margin:auto;display:block;float:center"> | ||
<p align="center"><img src="https://static.igem.org/mediawiki/2014/1/19/OliVEoil_Set.jpg" style="margin-left:auto;margin-right:auto;border:1.5px solid #212f20;"/></p> | <p align="center"><img src="https://static.igem.org/mediawiki/2014/1/19/OliVEoil_Set.jpg" style="margin-left:auto;margin-right:auto;border:1.5px solid #212f20;"/></p> | ||
Revision as of 02:53, 18 October 2014
With a working model, it was time for the ultimate test: Olive Oil
Nine samples of Extra Virgin Olive Oil were obtained and prepared for assay where three of the samples were known to be rancid. The velocities were recorded with each enzyme for a total of 27 data points. We used the best catalytic matrix from our previous model and again inverted the matrix and multiplied by the observed velocity. The results are below:
We noticed to outliers based on the unsaturated aldehyde prediction.
By plotting the predicted concentrations in three dimensions, we saw two obvious outliers. We deemed these two samples rancid based on their demarcation from the rest of the group, but also the high levels of both unsaturated aldehydes, and medium saturated aldehydes.
