Team:HokkaidoU Japan/Notebook/H Stem/Replacement To 1A3

From 2014.igem.org

(Difference between revisions)
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      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
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pSB1A3 with EcoRI & PstI,
+
pSB1A3 with EcoRI & PstI
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        R0010-stem-as-stem-B0015 with EcoRI & PstI
+
</div>
 +
<div class="step-recipe">
 +
R0010-stem-as-stem-B0015 with EcoRI & PstI
</div>
</div>
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      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
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0010-stem-as-stem-B0015 & pSB1A3
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Ligate 0010-stem-as-stem-B0015 with pSB1A3
</div>
</div>
    </div>
    </div>
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      <div class="step-recipe">
      <div class="step-recipe">
R0010-stem-as-stem-B0015(on pSB1A3)
R0010-stem-as-stem-B0015(on pSB1A3)
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        5.0&micro;L to DH5alpha;
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</div>
 +
<div class="step-recipe">
 +
5.0&micro;L to DH5alpha;
</div>
</div>

Revision as of 03:22, 18 October 2014

Notebook
Lab Documents

H-Stem Vector Exchange to pSB1A3

  • Start

  • Mini-Prep

    R0010-stem-as-stem-B0015 (on pSB1A3)

  • PCR

    R0010-stem-as-stem-B0015(on pSB1C3) with 100up_F Tm:62.6℃ & 200dn_R Tm:66.6℃

  • Digestion

    pSB1A3 with EcoRI & PstI
    R0010-stem-as-stem-B0015 with EcoRI & PstI

  • Ligation

    Ligate 0010-stem-as-stem-B0015 with pSB1A3

  • Transformation

    R0010-stem-as-stem-B0015(on pSB1A3)
    5.0µL to DH5alpha;

  • Complete!

Retrieved from "http://2014.igem.org/Team:HokkaidoU_Japan/Notebook/H_Stem/Replacement_To_1A3"