Team:Jilin China/Outline

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<h2>May to July, 2014</h2>
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<h2>May, 2014</h2>
<ol>
<ol>
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<li>MlrA gene coding optimization（codon of lactic acid bacteria）</li>
+
<li>Data query and project theme select </li>
-
<li>MlrA gene synthesis(synthesis by pieces)</li>
+
<li>Experiment scheme design </li>
 +
<li>Codon optimization of MlrA gene by lactic acid bacteria codons）</li>
 +
<li>MlrA gene sequence design and split</li>
 +
<li>RFP-Mlr-GFP sequence design and split</li>
 +
 
 +
<h2>June, 2014</h2>
 +
<ol>
 +
<li>MlrA gene synthesis and sequence</li>
 +
<li>RFP-Mlr-GFP gene synthesis and sequence</li>
 +
 
 +
 
 +
<h2>July, 2014</h2>
 +
<ol>
 +
<li>MlrA gene synthesis and sequence</li>
 +
<li>RFP-Mlr-GFP gene synthesis and sequence</li>
<li>Sequence analysis and discussion about whether it can be split</li>
<li>Sequence analysis and discussion about whether it can be split</li>
<li>Synthetic primer by company (33 pieces in total)</li>
<li>Synthetic primer by company (33 pieces in total)</li>

Revision as of 02:50, 18 October 2014

Welcome!
Team Jilin_China

May, 2014

  1. Data query and project theme select
  2. Experiment scheme design
  3. Codon optimization of MlrA gene by lactic acid bacteria codons）
  4. MlrA gene sequence design and split
  5. RFP-Mlr-GFP sequence design and split

    6. June, 2014

    1. MlrA gene synthesis and sequence
    2. RFP-Mlr-GFP gene synthesis and sequence

      3. July, 2014

      1. MlrA gene synthesis and sequence
      2. RFP-Mlr-GFP gene synthesis and sequence
      3. Sequence analysis and discussion about whether it can be split
      4. Synthetic primer by company (33 pieces in total)
      5. Repeat PCR for many times, recovery them and then get the complete gene product
      6. Sub cloning vector and sequenced
      7. Try to express by E.coli and analyze the effect of this protein
      8. Express by lactic acid bacteria

      July to Sept, 2014

      1. Discussion about many possible paths include MC-LR
      2. Try cloning Pseudomonas natural promoter.(non-coding sequences in Mlr enzyme series )
      3. Synthesis by pieces then got complete product.
      4. The gene transformed into E.coli and verify its functions.
      5. Transform this gene by many ways into lactic acid bacteria and verify the function of promoter.
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