Team:British Columbia/Notebook/Protocols

From 2014.igem.org

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Restriction digest with BioBrick plasmid an inserts
Restriction digest with BioBrick plasmid an inserts
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/ligation">BioBrick Ligation</a> </font> </b>  
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<li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/ligation">BioBrick Ligation</a> </font> </b>  
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Ligate Biobrick insert to vector
Ligate Biobrick insert to vector
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/plates">Making Plates</a> </font> </b>  
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<li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/plates">Making Plates</a> </font> </b>  
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Make LB plates with antibiotics
Make LB plates with antibiotics
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/media">Liquid media</a> </font> </b>  
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<li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/media">Liquid media</a> </font> </b>  
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Make liquid LB media
Make liquid LB media
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/compcells">Competent cells</a> </font> </b>  
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<li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/compcells">Competent cells</a> </font> </b>  
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Preparation of chemically competent cells
Preparation of chemically competent cells
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/transformation">Transformation</a> </font> </b>  
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<li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/transformation">Transformation</a> </font> </b>  
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Transformation of plasmi DNA into chemically competent cells
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Transformation of plasmid DNA into chemically competent cells
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/RepeatSpacerAssembly ">CRISPR Repeat-Spacer Array Assembly</a> </font> </b>
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<li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/AnnealedOligonucleotides">Assembling Parts from Oligonucleotides</a> </font> </b>  
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Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/AnnealedOligonucleotides">Assembling Parts from Oligonucleotides</a> </font> </b>  
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Assemble short parts from annealed oligonucleotides
Assemble short parts from annealed oligonucleotides
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/MultisiteMutagenesis">(Multi) Site Directed Mutagenesis</a> </font> </b>  
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<li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/MultisiteMutagenesis">(Multi) Site Directed Mutagenesis</a> </font> </b>  
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Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.
Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/T4PNK">Oligonucleotide Phosphorylation</a> </font> </b>  
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<li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/T4PNK">Oligonucleotide Phosphorylation</a> </font> </b>  
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Phosphorylate oligonucleotides prior to ligation
Phosphorylate oligonucleotides prior to ligation
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/ElectrocompCells">Electrocompetent Cell Production</a> </font> </b>  
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<li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/ElectrocompCells">Electrocompetent Cell Production</a> </font> </b>  
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Make electrocompetent cells
Make electrocompetent cells
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<li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/CombinatorialLibraries">Make Combinatorial Part Libraries</a> </font> </b>
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Make combinatorial part libraries from compatible parts
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</ul>
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Revision as of 02:42, 18 October 2014

2014 UBC iGEM