Team:British Columbia/Notebook/Protocols
From 2014.igem.org
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Restriction digest with BioBrick plasmid an inserts | Restriction digest with BioBrick plasmid an inserts | ||
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- | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/ligation">BioBrick Ligation</a> </font> </b> | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/ligation">BioBrick Ligation</a> </font> </b> |
</li> | </li> | ||
Ligate Biobrick insert to vector | Ligate Biobrick insert to vector | ||
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- | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/plates">Making Plates</a> </font> </b> | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/plates">Making Plates</a> </font> </b> |
</li> | </li> | ||
Make LB plates with antibiotics | Make LB plates with antibiotics | ||
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- | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/media">Liquid media</a> </font> </b> | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/media">Liquid media</a> </font> </b> |
</li> | </li> | ||
Make liquid LB media | Make liquid LB media | ||
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- | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/compcells">Competent cells</a> </font> </b> | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/compcells">Competent cells</a> </font> </b> |
</li> | </li> | ||
Preparation of chemically competent cells | Preparation of chemically competent cells | ||
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- | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/transformation">Transformation</a> </font> </b> | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/transformation">Transformation</a> </font> </b> |
</li> | </li> | ||
- | Transformation of | + | Transformation of plasmid DNA into chemically competent cells |
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- | <li> <b><font size="5"><a href=" | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/AnnealedOligonucleotides">Assembling Parts from Oligonucleotides</a> </font> </b> |
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</li> | </li> | ||
Assemble short parts from annealed oligonucleotides | Assemble short parts from annealed oligonucleotides | ||
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- | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/MultisiteMutagenesis">(Multi) Site Directed Mutagenesis</a> </font> </b> | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/MultisiteMutagenesis">(Multi) Site Directed Mutagenesis</a> </font> </b> |
</li> | </li> | ||
Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations. | Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations. | ||
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- | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/T4PNK">Oligonucleotide Phosphorylation</a> </font> </b> | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/T4PNK">Oligonucleotide Phosphorylation</a> </font> </b> |
</li> | </li> | ||
Phosphorylate oligonucleotides prior to ligation | Phosphorylate oligonucleotides prior to ligation | ||
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- | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/ElectrocompCells">Electrocompetent Cell Production</a> </font> </b> | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/ElectrocompCells">Electrocompetent Cell Production</a> </font> </b> |
</li> | </li> | ||
Make electrocompetent cells | Make electrocompetent cells | ||
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</ul> | </ul> |
Revision as of 02:42, 18 October 2014
Protocols
- PCR Instruction for colony PCR and regular PCR
- Gel Verification Make a gel and conduct gel elecrophoresis
- BioBrick Restriction Digest Restriction digest with BioBrick plasmid an inserts
- BioBrick Ligation Ligate Biobrick insert to vector
- Making Plates Make LB plates with antibiotics
- Liquid media Make liquid LB media
- Competent cells Preparation of chemically competent cells
- Transformation Transformation of plasmid DNA into chemically competent cells
- Assembling Parts from Oligonucleotides Assemble short parts from annealed oligonucleotides
- (Multi) Site Directed Mutagenesis Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.
- Oligonucleotide Phosphorylation Phosphorylate oligonucleotides prior to ligation
- Electrocompetent Cell Production Make electrocompetent cells