Team:EPF Lausanne/Parts

From 2014.igem.org

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   <tr>
   <tr>
     <td class="biobrick_name">BBa_K1486013</td>
     <td class="biobrick_name">BBa_K1486013</td>
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     <td>CpxR + IFP[2]</td>
+
     <td>CpxR-IFP[2] under pBAD</td>
     <td>CpxR with the Cterm moiety of IFP attached at its C terminus</td>
     <td>CpxR with the Cterm moiety of IFP attached at its C terminus</td>
     <td>Intermediate & control</td>
     <td>Intermediate & control</td>
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     <td class="biobrick_name">BBa_K1486014</td>
     <td class="biobrick_name">BBa_K1486014</td>
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     <td>IFP[1] + CpxR</td>
+
     <td>IFP[1]-CpxR under pBAD</td>
     <td>CpxR with the Nterm moiety of IFP attached at its N terminus</td>
     <td>CpxR with the Nterm moiety of IFP attached at its N terminus</td>
     <td>Intermediate & control</td>
     <td>Intermediate & control</td>

Revision as of 23:35, 17 October 2014

PARTS


DNA parts submitted by the 2014 EPFL iGEM team

Our team submitted a total of 55 Biobricks (part 51 does not exist).

In addition, 4 microfluidic designs have also been submitted to the registry.

Biobrick What it is Function Why do we use it? Group
BBa_K1486000 CpxR coding sequence Transcription factor To make most of our biobricks! Bacteria
BBa_K1486001 CpxR + arabinose promoter Transcription factor Bacteria
BBa_K1486002 Arabinose promoter + sfGFP CpxR [Nterm] Expresses fused protein Test CpxR expression & Ara promoter Bacteria
BBa_K1486003 Flexible linker 2x (GGGS) Attaches two proteins together Bacteria
BBa_K1486004 Flexible linker 2x (GGGGS) Attaches two proteins together Bacteria
BBa_K1486005 Ara promoter + CpxR sfGFP [Cterm] Expresses fused protein Test CpxR expression & Ara promoter Bacteria
BBa_K1486006 IFP[1] N terminus of split IFP Bacteria
BBa_K1486007 IFP[2] C terminus of split IFP Bacteria
BBa_K1486008 CxpR & Split IFP1.4 [Cterm + Cterm] Two CpxR CDS, each C terminus attached to a moiety of IFP Characterize CpxR dimerization Bacteria
BBa_K1486009 CxpR & Split IFP1.4 [Nterm + Nterm] Two CpxR CDS, each N terminus attached to a moiety of IFP Characterize CpxR dimerization Bacteria
BBa_K1486010 CxpR & Split IFP1.4 [Nterm + Cterm] Two CpxR CDS, each attached to a moiety of IFP Characterize CpxR dimerization Bacteria
BBa_K1486011 CxpR & Split IFP1.4 [Cterm + Nterm] Two CpxR CDS, each attached to a moiety of IFP Characterize CpxR dimerization Bacteria
BBa_K1486012 CpxR-IFP[1] under pBAD] CpxR with the Nterm moiety of IFP attached at its C terminus Intermediate & control Bacteria
BBa_K1486013 CpxR-IFP[2] under pBAD CpxR with the Cterm moiety of IFP attached at its C terminus Intermediate & control Bacteria
BBa_K1486014 IFP[1]-CpxR under pBAD CpxR with the Nterm moiety of IFP attached at its N terminus Intermediate & control Bacteria
BBa_K1486015 IFP[2] + CpxR CpxR with the Cterm moiety of IFP attached at its N terminus Intermediate & control Bacteria
BBa_K1486016 fLuc[1] N terminus moiety of the firefly luciferase Bacteria
BBa_K1486017 fLuc[2] C terminus moiety of the firefly luciferase Bacteria
BBa_K1486018 PAra + fLuc[1] + fLuc[2] Split firefly luciferase under arabinose promoter Control Bacteria
BBa_K1486019 rLuc[1] C terminus moiety of the renilla luciferase Bacteria
BBa_K1486020 rLuc[2] N terminus moiety of the renilla luciferase Bacteria
BBa_K1486021 PAra + rLuc[1] + rLuc[2] Split renilla luciferase under arabinose promoter Control Bacteria
BBa_K1486022 rLuc Full renilla luciferase Control Bacteria
BBa_K1486023 yeast sfGFP Yeast optimised superfolder GFP Reporter Yeast
BBa_K1486024 Kan Yeast kanamycin resistance Selection marker Yeast
BBa_K1486025 ADH1 terminator Terminator Abortion of the transcription at the end of our DNA sequences Yeast
BBa_K1486026 sfGFP + ADH1+ Kan tag Control for the expression of Pbs2 Yeast
BBa_K1486027 rLuc + ADH1 + Kan tag Control for the expression of Pbs2 Yeast
BBa_K1486028 Yeast sfGFP[1] Yeast optimized sfGFP N-terminus (1-214) Yeast
BBa_K1486029 sfGFP[1] + ADH1 + kan N terminal moiety of split sfGFP Complementation Assay Yeast
BBa_K1486030 rLuc[1] + ADH1 + kan N terminal moiety of split rLuc Complementation Assay Yeast
BBa_K1486031 Ura3 cassette selection marker Confer resistance to Uracil-deprived medium Yeast
BBa_K1486032 sfGFP + ADH1 terminator + Ura3 cassette tag Control for the expression of hog1 Yeast
BBa_K1486033 rLuc + ADH1 + Ura tag Control for the expression of hog1 Yeast
BBa_K1486034 yeast sfGFP[2] Yeast optimized superfolder GFP C-terminus (215-238) Yeast
BBa_K1486035 yeast sfGFP[2]+ ADH1 + Ura C terminal moiety of split sfGFP Complementation Assay Yeast
BBa_K1486036 rLuc[2] + ADH1 + Ura C terminal moiety of split rLuc Complementation Assay Yeast
BBa_K1486037 13 amino acids linker [GGGS GGGGS GGGS] linker Attach Pbs2 or Hog1 to our different tags and splits Yeast
BBa_K1486038 sfGFP[1] N terminus moiety of split superfolder GFP Bacteria
BBa_K1486039 sfGFP[2] C terminus moiety of split superfolder GFP Bacteria
BBa_K1486040 sfGFP[1] + CpxR N terminus moiety of split sfGFP attached to CpxR Bacteria
BBa_K1486041 sfGFP[2] + CpxR C terminus moiety of split sfGFP attached to CpxR Bacteria
BBa_K1486042 LZip Monomer of leucine zipper TF Bacteria
BBa_K1486043 LZip + split rLuc Two Leucine Zipper monomers, each attached to a different split rLuc moiety Control for split rLuc assays Bacteria
BBa_K1486044 mut IFP[1] Biobrick-compatible IFP[1] Bacteria
BBa_K1486045 mut IFP[2] Biobrick-compatible IFP[2] Bacteria
BBa_K1486046 CpxR promoter FW CpxR binding-region in forward direction Bacteria
BBa_K1486047 CpxR promoter RV CpxR binding-region in reverse direction Bacteria
BBa_K1486048 CpxR reporter Calgary's CpxR reporter repaired (sequence was missing) To see when CpxR is active Bacteria
BBa_K1486049 CpxR promoter FW + RFP Reporter of CpxR Test the direction of the complete CpxR promoter Bacteria
BBa_K1486050 CpxR promoter RV + RFP Reporter of CpxR Test the direction of the complete CpxR promoter Bacteria
BBa_K1486052 Spacer 40 bases placed between constructs Separate two constructs in the same plasmid Bacteria
BBa_K1486053 Linker 10 amino-acid linker Attach CheY/Z to split luciferases Bacteria
BBa_K1486054 CheY/CheZ rLuc CheY and CheZ, each attached to a moiety of split renilla luciferase Positive control for the split rLuc Bacteria
BBa_K1486055 CheY/CheZ fLuc CheY and CheZ, each attached to a moiety of split firefly luciferase Positive control for the split fLuc Bacteria
BBa_K1486056 CxpR & Split mut IFP1.4 [Cterm + Cterm] Two CpxR CDS, each C terminus attached to a moiety of the biobrick-compatible IFP Characterize CpxR dimerization Bacteria


Microfluidics parts (chips created)

Our team designed and made 4 microfluidic chips. At the beginning, we also used the MITOMI chip.

When designing the chips, the team took into account the future users and the current iGEM classification of parts. We considered it best to construct our chips as composite microfluidic parts, so their sub-parts could be used and combined in multiple ways. The flow and control layers can be separated and reused, as well as all the basic structures (chamber + connecting channel), nodes, array parts,...

Name Main Function
SmashColi To be able to separate the chip in 4 different compartments and apply 4 different pressures on each row of chambers.
The BioPad A large and simple microfluidic chip containing 6400 chambers in which the cells are contained in. Each chamber acts as a pixel for the BioPad project.
CleanColi As a result of our Safety page, we decided to create a chip that is able to seal the bacteria in the chip, preventing them to leave the chip.
FilterColi To successfully immerse cells in a certain solution, this chip was designed to flow in the new medium in the chambers instead of doing it by diffusion.


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