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Team:UI-Indonesia/Parts/Characterization
From 2014.igem.org
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<b>Background</b><br> | <b>Background</b><br> | ||
Peptide 1018 is small cationic peptide under T5 promoter (inducible promoter). Peptide 1018 in particular concentration can be toxic for bacterial vector. Isopropyl thiogalactoside (IPTG) is analogue of lactose that is able to induce activation of T5 promoter to start transcription. | Peptide 1018 is small cationic peptide under T5 promoter (inducible promoter). Peptide 1018 in particular concentration can be toxic for bacterial vector. Isopropyl thiogalactoside (IPTG) is analogue of lactose that is able to induce activation of T5 promoter to start transcription. | ||
| - | <br> | + | <br><br> |
<b>Aim</b><br> | <b>Aim</b><br> | ||
To examine in which concentration of IPTG that can be toxic for E.coli as bacterial vector | To examine in which concentration of IPTG that can be toxic for E.coli as bacterial vector | ||
| - | <br> | + | <br><br> |
<b>Result</b><br> | <b>Result</b><br> | ||
<img src="https://static.igem.org/mediawiki/2014/9/90/Antimicrobial_result.png" width="auto" height="auto"> | <img src="https://static.igem.org/mediawiki/2014/9/90/Antimicrobial_result.png" width="auto" height="auto"> | ||
Revision as of 22:42, 17 October 2014
Peptide 1018 Antimicrobial Assay
Background
Peptide 1018 is small cationic peptide under T5 promoter (inducible promoter). Peptide 1018 in particular concentration can be toxic for bacterial vector. Isopropyl thiogalactoside (IPTG) is analogue of lactose that is able to induce activation of T5 promoter to start transcription.
Aim
To examine in which concentration of IPTG that can be toxic for E.coli as bacterial vector
Result
