Team:Groningen/Template/MODULE/Notebook/bandage/week14

From 2014.igem.org

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This week new cultures were grown for freeze drying. This was done to test the growth in chemically defined medium (CDM), which is the medium that will be used in the final bandage. Cultures with several OD<sub>600</sub> values were freeze dried in 5 ml samples. Unfortunately the freeze drying machine could only be used on the 9<sup>th</sup> of October. Samples were taken from the machine on the 10<sup>th</sup> of October and one of the samples was resuspended in 1 ml demi water to check for viability. The resuspended cells were inoculated in CDM in a 1:100 ratio and grown overnight at 200 rpm and 30 C.
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New <i>L. lactis</i> NZ9000 with pNZ8048g were grew overnight in Chemically Defined Medium (CDM), and freeze dried the next day for several OD<sub>600</sub> values
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Growth was visible in the medium the next morning. This means the cells were viable and therefore the freeze drying of <i>Lactococcus lactis</i> was successful.
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One of the freeze dried samples was resuspended in 1 mL demi water to check for viability
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The resuspended cells were inoculated in CDM in a 1:100 ratio and grown overnight at 200 rpm and 30 C
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This week new cultures were grown for freeze drying. This was done to test the growth in chemically defined medium (CDM), which is the medium that will be used in the final bandage. Cultures with several OD<sub>600</sub> values were freeze dried in 5 ml samples. Unfortunately the freeze drying machine could only be used on the 9<sup>th</sup> of October. Samples were taken from the machine on the 10<sup>th</sup> of October and one of the samples was resuspended in 1 ml demi water to check for viability. The resuspended cells were inoculated in CDM in a 1:100 ratio and grown overnight at 200 rpm and 30 °C
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Latest revision as of 03:31, 18 October 2014

October 6 - October 10
 
New L. lactis NZ9000 with pNZ8048g were grew overnight in Chemically Defined Medium (CDM), and freeze dried the next day for several OD600 values
 
One of the freeze dried samples was resuspended in 1 mL demi water to check for viability
 
The resuspended cells were inoculated in CDM in a 1:100 ratio and grown overnight at 200 rpm and 30 C
 
This week new cultures were grown for freeze drying. This was done to test the growth in chemically defined medium (CDM), which is the medium that will be used in the final bandage. Cultures with several OD600 values were freeze dried in 5 ml samples. Unfortunately the freeze drying machine could only be used on the 9th of October. Samples were taken from the machine on the 10th of October and one of the samples was resuspended in 1 ml demi water to check for viability. The resuspended cells were inoculated in CDM in a 1:100 ratio and grown overnight at 200 rpm and 30 °C
 
 
 
 

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