"
Team:Bordeaux/NotebookJune11
From 2014.igem.org
(Difference between revisions)
| Line 8: | Line 8: | ||
<ul class="day-detail"> | <ul class="day-detail"> | ||
| - | <li>Preparation of vector pSB1C3 for ligation with our PCR products (VPGXG-20, VPGXG-40 and VPGXG-60) : | + | <li>Preparation of vector pSB1C3 for ligation with our PCR products (VPGXG-20, VPGXG-40 and VPGXG-60) : digestion, ligation and transformation </li><br> |
<li>Making LB+Chlo and LB+Amp plates</li></br> | <li>Making LB+Chlo and LB+Amp plates</li></br> | ||
<li>Making competent cells: day 2</li> | <li>Making competent cells: day 2</li> | ||
Latest revision as of 21:37, 17 October 2014
11th June
Molecular biology
- Preparation of vector pSB1C3 for ligation with our PCR products (VPGXG-20, VPGXG-40 and VPGXG-60) : digestion, ligation and transformation
- Making LB+Chlo and LB+Amp plates
- Making competent cells: day 2
Production
- 30mL preculture of LB + Yeast Extract 5g/L + glycerol 1g/L + Amp at 37°C overnight
- VPGXG-20
- VPGXG-40
- 30mL preculture of LB + Yeast Extract 5g/L + glucose 1g/L + Amp at 37°C overnight
- VPGXG-60
Purification
- End of purification protocol and store at 4°C.
