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From 2014.igem.org

11^th June

• Preparation of vector pSB1C3 for ligation with our PCR products (VPGXG-20, VPGXG-40 and VPGXG-60) : digestion, ligation and transformation


• Making LB+Chlo and LB+Amp plates


• Making competent cells: day 2

• 30mL preculture of LB + Yeast Extract 5g/L + glycerol 1g/L + Amp at 37°C overnight
• VPGXG-20
• VPGXG-40
• 30mL preculture of LB + Yeast Extract 5g/L + glucose 1g/L + Amp at 37°C overnight
• VPGXG-60

• End of purification protocol and store at 4°C.