Team:Hong Kong HKUST/riboregulator/parts

From 2014.igem.org

(Difference between revisions)
Line 128: Line 128:
<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_I0500">BBa_I0500</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_I0500">BBa_I0500</a></td>  
-
  <td>Inducible pBad/araC promoter</td>       
+
  <td>Regulatory</td>       
  <td>Inducible pBad/araC promoter</td>    
  <td>Inducible pBad/araC promoter</td>    
  <td>1210</td>
  <td>1210</td>
Line 135: Line 135:
<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J01010">BBa_J01010</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J01010">BBa_J01010</a></td>  
-
  <td>Riboregulator Lock 1</td>       
+
  <td>RNA</td>       
-
  <td>Biobrick version of Isaacs' riboregulator cis repressed lock, crR12.</td>    
+
  <td>Riboregulator Lock 1</td>    
  <td>40</td>
  <td>40</td>
</tr>
</tr>
Line 142: Line 142:
<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J01080">BBa_J01080</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J01080">BBa_J01080</a></td>  
-
  <td>Lock3</td>       
+
  <td>RNA</td>       
-
  <td>Biobrick version of Isaacs' riboregulator cis repressed lock, crR12, and modified version of Riboregulator Lock 1.</td>    
+
  <td>Lock3</td>    
  <td>40</td>
  <td>40</td>
</tr>
</tr>
Line 149: Line 149:
<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J01086">BBa_J01086</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J01086">BBa_J01086</a></td>  
-
  <td>Key3</td>       
+
  <td>RNA</td>       
-
  <td>Biobricked version of Isaacs' riboregulator trans activating key, taR12, and modified version of Riboregulator Key 1.</td>    
+
  <td>Key3</td>    
  <td>94</td>
  <td>94</td>
</tr>
</tr>
Line 156: Line 156:
<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K175031">BBa_K175031</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K175031">BBa_K175031</a></td>  
-
  <td>Lock for medium RBS (B0032) from the lock/key library TUD09</td>     
+
  <td>RNA</td>     
-
  <td>This biobrick generates a mRNA which forms a secondary structure blocking the (medium) RBS.</td>    
+
  <td>Lock for medium RBS (B0032) from the lock/key library TUD09</td>    
  <td>47</td>
  <td>47</td>
</tr>
</tr>
Line 163: Line 163:
<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K175032">BBa_K175032</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K175032">BBa_K175032</a></td>  
-
  <td>Key for lock of medium (K175031) RBS (B0032) from the lock/key library TUD09</td>     
+
  <td>RNA</td>     
-
  <td>This biobrick generates a mRNA which forms a secondary structure that opens LMR (K175031).</td>    
+
  <td>Key for lock of medium (K175031) RBS (B0032) from the lock/key library TUD09</td>    
  <td>84</td>
  <td>84</td>
</tr>
</tr>
Line 170: Line 170:
<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J23080">BBa_J23080</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J23080">BBa_J23080</a></td>  
-
  <td>B0034 derived RBS to match spacing of J01122</td>       
+
  <td>RBS</td>       
-
  <td>RBS spacing modification to [RBS] such that the spacing between the shine-delgarno and the ORF is the same as it is in J01122.</td>    
+
  <td>B0034 derived RBS to match spacing of J01122</td>    
  <td>10</td>
  <td>10</td>
</tr>
</tr>
Line 194: Line 194:
<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J01008">BBa_J01008</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J01008">BBa_J01008</a></td>  
-
  <td>Riboregulator Lock 1</td>       
+
  <td>RNA</td>       
-
  <td>Lock 1</td>    
+
  <td>Riboregulator key 1</td>    
  <td>94</td>
  <td>94</td>
</tr>
</tr>
Line 201: Line 201:
<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J23031">BBa_J23031</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J23031">BBa_J23031</a></td>  
-
  <td>Lock 3c</td>       
+
  <td>RNA</td>       
  <td>lock3c</td>    
  <td>lock3c</td>    
  <td>42</td>
  <td>42</td>
Line 208: Line 208:
<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J23008">BBa_J23008</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J23008">BBa_J23008</a></td>  
-
  <td>Key 3c</td>       
+
  <td>RNA</td>       
-
  <td>Encodes a short RNA sequence that "unlocks" the biobrick part J01122 (lock3) which is locked RFP.</td>    
+
  <td>key3c</td>    
  <td>42</td>
  <td>42</td>
</tr>
</tr>

Revision as of 20:46, 17 October 2014



Riboregulator Parts

Parts that we designed and submitted

Name Type Description Length
BBa_K1379015 RBS RBS(Lock 3c) 12
BBa_K1379018 RNA HKUST Lock 1 45
BBa_K1379019 RNA HKUST Key 1 82
BBa_K1379020 Intermediate Lock 1-GFP-B0015 903
BBa_K1379022 Intermediate Lock 3-GFP-B0015 905
BBa_K1379024 Intermediate Lock 3c-GFP-B0015 905
BBa_K1379026 Intermediate K175031-GFP-B0015 910
BBa_K1379028 Intermediate HKUST Lock 1-GFP-B0015 908
BBa_K1379046 Intermediate RBS(Lock 1)-GFP-B0015 873
BBa_K1379047 Intermediate RBS(Lock 3c)-GFP-B0015 875
BBa_K1379048 Measurement J23102-Lock 1-GFP-B0015-pBad/araC-Key 1-B0015 2403


Parts that we constructed and submitted

Name Type Description Length
BBa_I0500 Regulatory Inducible pBad/araC promoter 1210
BBa_J01010 RNA Riboregulator Lock 1 40
BBa_J01080 RNA Lock3 40
BBa_J01086 RNA Key3 94
BBa_K175031 RNA Lock for medium RBS (B0032) from the lock/key library TUD09 47
BBa_K175032 RNA Key for lock of medium (K175031) RBS (B0032) from the lock/key library TUD09 84
BBa_J23080 RBS B0034 derived RBS to match spacing of J01122 10


Parts that already exist in Parts Registry and are currently under characterization

Name Type Description Length
BBa_J01008 RNA Riboregulator key 1 94
BBa_J23031 RNA lock3c 42
BBa_J23008 RNA key3c 42


Parts that we designed but did not submit

Name Type Description Length
BBa_K1379021 Intermediate Key 1-B0015 231
BBa_K1379023 Intermediate Key 3-B0015 231
BBa_K1379025 Intermediate Key 3c-B0015 231
BBa_K1379027 Intermediate K175032-B0015 221
BBa_K1379029 Intermediate HKUST Key 1-B0015 219
BBa_K1379030 Measurement J23102-Lock 1-GFP-B0015 946
BBa_K1379031 Intermediate pBad/araC-Key 1 1312
BBa_K1379032 Measurement J23102-Lock 3-GFP-B0015 948
BBa_K1379033 Intermediate pBad/araC-Key 3 1312
BBa_K1379034 Measurement J23102-Lock 3c-GFP-B0015 948
BBa_K1379035 Intermediate pBad/araC-Key 3c 1312
BBa_K1379036 Measurement J23102-K175031-GFP-B0015 953
BBa_K1379037 Intermediate pBad/araC-K175032 1302
BBa_K1379038 Measurement J23102-HKUST Lock 1-GFP-B0015 951
BBa_K1379039 Intermediate pBad/araC-HKUST Key 1 1300

Home

Pneumosensor

Riboregulator

Human Practice

Team

WetLab

Achievement

Retrieved from "http://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/parts"