Team:SCU-China/Modeling

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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:SCU-China/Modeling&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=SCU-China"style="color:#000000"> Official Team Profile </a></td>
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                <li><a href="https://2014.igem.org/Team:SCU-China/UESTC">Visiting University of Electronic Science and Technology of China (UESTC)</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Biobricks">Notebook of Biobricks</a></li>
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                <li class="dropdown-header">Method</li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Prep">Bacterial Genomic DNA Prep</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Digestion">Digestion</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/GelExtraction">Gel Extraction </a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Linkage">Linkage</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/PCR">PCR</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Plasmid">Plasmid Mini Prep</a></li>
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<p>According to the demand of IGEM Competition, we usually use the EcoR I, Xba I, Spe I, and Pst I to do the digestion and the following is our system (20&#956;l).</p>
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<table class="table table-striped"><tr><td><p>DNA</p>
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</td><td><p>250ng</p>
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</tr><tr><td><p>Enzyme 1</p>
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</td><td><p>1&#956;l</p>
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</tr><tr><td><p>10 X Buffer</p>
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</td><td><p>2&#956;l</p>
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</tr><tr><td><p>H2O</p>
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</td><td><p>Up to 20&#956;l</p>
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<p>If you choose to create a model during your project, please write about it here. Modeling is not an essential part of iGEM, but we encourage any and all teams to model some aspect of their project. See previous "Best Model" awards for more information.</p>
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  <p>Sichuan university</p>
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Revision as of 22:16, 17 October 2014

Modeling

According to the demand of IGEM Competition, we usually use the EcoR I, Xba I, Spe I, and Pst I to do the digestion and the following is our system (20μl).

DNA

250ng

Enzyme 1

1μl

Enzyme 2

1μl

10 X Buffer

2μl

H2O

Up to 20μl

Sichuan university

Retrieved from "http://2014.igem.org/Team:SCU-China/Modeling"