Team:Caltech/Project/Methods and Methods
From 2014.igem.org
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+ | <h4>Transformations</h4> | ||
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+ | <b> For JM109 cells: </b> | ||
+ | <ul><li> Add 2 uL of plasmid to 20 uL of cells</li> | ||
+ | <li> Stir with pipette tip</li> | ||
+ | <li> Keep cells on ice</li> | ||
+ | <li> Plate with glass beads </li> | ||
+ | </ul> | ||
+ | <b> For DH5& alpha Z1 cells:</b> | ||
+ | <ul><li> Add 1 uL of plasmid to 40 uL of cells </li> | ||
+ | <li> Incubate on ice for 10 minutes </li> | ||
+ | <li> Heat shock at 42C for 90 seconds </li> | ||
+ | <li> Add 100 uL of SOC medium, then incubate in a 37C shaker for 30-90 minutes to recover </li> | ||
+ | <li> Plate with glass beads </li> | ||
+ | </ul> | ||
+ | </td> | ||
+ | </tr> | ||
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Revision as of 21:39, 14 July 2014
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Overall Project Summary
Project Details Materials and Methods The Experiments Results Data Analysis Conclusions References |
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