Team:TU Darmstadt/Notebook/Methods/Dichloromethane extraction

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<!--TYPO3SEARCH_begin--><div id="c247" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">Dichloromethane extraction</h1></div><div class="csc-textpic-text"><div><p><b>Materials:&nbsp;</b></p></div><div><p>- Dichloromethane</p></div><div><p>- 37% hydrochloric acid</p></div><div><p>- Centrifuge</p></div><div><p>- Tubes</p></div><div></div><div><p><b>Procedure:</b></p></div><div><p>Spin down the bacterial solution at 4500g for 10 minutes.</p></div><div><p>Remove culture media, weigh the remaining pellet, add the same amount of dichloromethane and vortex for 5 minutes. Separate supernatant and pellet and acetify each with about 300µl of 37% hydrochloric acid till the color turns to red.&nbsp;</p></div><div><p>Spin down the resolved pellet at 13,3g for 10 minutes and Remove supernatant.</p></div><div><p>The anthocyanidin is present in the organic and the aqueous phase; the color can be changed by adjusting the pH-value.&nbsp;</p></div><div></div></div></div><!--TYPO3SEARCH_end-->
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<!--TYPO3SEARCH_begin--><div id="c247" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">Dichloromethane extraction</h1></div><div class="csc-textpic-text"><div><p><b>Materials:&nbsp;</b></p></div><div><p>- Dichloromethane</p></div><div><p>- 37% hydrochloric acid</p></div><div><p>- Centrifuge</p></div><div><p>- Tubes</p></div><div></div><div><p><b>Procedure:</b></p></div><div><p>- Spin down the bacterial solution at 4500g for 10 minutes.</p></div><div><p>- Remove culture media, weigh the remaining pellet, add the same amount of dichloromethane and vortex for 5 minutes. Separate supernatant and pellet and acetify each with about 300µl of 37% hydrochloric acid till the color turns to red.&nbsp;</p></div><div><p>- Spin down the resolved pellet at 13,3g for 10 minutes and Remove supernatant.</p></div><div><p>The anthocyanidin is present in the organic and the aqueous phase; the color can be changed by adjusting the pH-value.&nbsp;</p></div><div></div></div></div><!--TYPO3SEARCH_end-->
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Revision as of 18:51, 17 October 2014

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Dichloromethane extraction

Materials: 

- Dichloromethane

- 37% hydrochloric acid

- Centrifuge

- Tubes

Procedure:

- Spin down the bacterial solution at 4500g for 10 minutes.

- Remove culture media, weigh the remaining pellet, add the same amount of dichloromethane and vortex for 5 minutes. Separate supernatant and pellet and acetify each with about 300µl of 37% hydrochloric acid till the color turns to red. 

- Spin down the resolved pellet at 13,3g for 10 minutes and Remove supernatant.

The anthocyanidin is present in the organic and the aqueous phase; the color can be changed by adjusting the pH-value.